使用响应面法预测电铸 giant unilamellar vesicle 的大小。
Prediction of the size of electroformed giant unilamellar vesicle using response surface methodology.
机构信息
State Key Laboratory of Urban Water Resource and Environment, School of Chemical Engineering and Technology, Harbin Institute of Technology, 92West Da-Zhi Street, Harbin 150001, China.
State Key Laboratory of Urban Water Resource and Environment, School of Chemical Engineering and Technology, Harbin Institute of Technology, 92West Da-Zhi Street, Harbin 150001, China.
出版信息
Biophys Chem. 2019 Oct;253:106217. doi: 10.1016/j.bpc.2019.106217. Epub 2019 Jul 4.
The production of giant unilamellar vesicles (GUVs) with specific size and structure has been a challenge on the design of quantitative biological assays in cell-mimetic micro-compartments. In this study, the effect of electroformation parameters (electric potential, frequency, and temperature) on the size of GUVs was investigated. Using response surface methodology based on Box-Behnken design, GUVs from neutral, positive and negative charges were formulated. The average diameter of GUVs was determined for each formulation. The acquired data of these GUVs were successfully fitted with quadratic regression models. These models were applied to visualize the parameters for ideal GUVs with wanted diameters by the obtained phase diagrams. These results show that response surface methodology can be used to estimate the electroformation parameters for specifically sized GUVs.
制备具有特定大小和结构的巨型单室囊泡（GUV）一直是在细胞模拟微区室中设计定量生物学测定的挑战。在这项研究中，研究了电成形参数（电势、频率和温度）对 GUV 大小的影响。使用基于 Box-Behnken 设计的响应面方法，制备了带正电荷、负电荷和中性电荷的 GUV。为每个配方确定了 GUV 的平均直径。用二次回归模型对这些 GUV 的获得的数据进行拟合。这些模型可用于通过获得的相图可视化具有所需直径的理想 GUV 的参数。这些结果表明，可以使用响应面方法来估计特定大小的 GUV 的电成形参数。
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