扫描荧光相关光谱学圆满完成。
Scanning fluorescence correlation spectroscopy comes full circle.
机构信息
Laboratorio de Cinética y Fotoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile; Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA; Universidad de Concepción, Facultad de Ciencias Químicas, Departamento de Polímeros, Concepción, Chile.
Laboratorio de Cinética y Fotoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile; Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA; Universidad de Concepción, Facultad de Ciencias Químicas, Departamento de Polímeros, Concepción, Chile.
出版信息
Methods. 2018 May 1;140-141:52-61. doi: 10.1016/j.ymeth.2018.01.023. Epub 2018 Feb 7.
In this article, we review the application of fluorescence correlation spectroscopy (FCS) methods to studies on live cells. We begin with a brief overview of the theory underlying FCS, highlighting the type of information obtainable. We then focus on circular scanning FCS. Specifically, we discuss instrumentation and data analysis and offer some considerations regarding sample preparation. Two examples from the literature are discussed in detail. First, we show how this method, coupled with the photon counting histogram analysis, can provide information on yeast ribosomal structures in live cells. The combination of scanning FCS with dual channel detection in the study of lipid domains in live cells is also illustrated.
在本文中，我们回顾了荧光相关光谱（FCS）方法在活细胞研究中的应用。我们首先简要介绍了 FCS 的理论基础，重点介绍了可获得的信息类型。然后，我们专注于环形扫描 FCS。具体来说，我们讨论了仪器和数据分析，并提供了一些关于样品制备的考虑因素。详细讨论了文献中的两个示例。首先，我们展示了如何将这种方法与光子计数直方图分析相结合，提供活细胞中酵母核糖体结构的信息。还说明了将扫描 FCS 与双通道检测相结合用于研究活细胞中脂质域的方法。
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