逆荧光相关光谱学的潜力与陷阱。

Potentials and pitfalls of inverse fluorescence correlation spectroscopy.

机构信息

SciLifeLab, Royal Institute of Technology, Department of Applied Physics, Experimental Biomolecular Physics Group, Solna 171 65, Sweden.

出版信息

Methods. 2018 May 1;140-141:23-31. doi: 10.1016/j.ymeth.2018.01.005. Epub 2018 Feb 2.

DOI:10.1016/j.ymeth.2018.01.005

PMID:29397309

Abstract

Inverse Fluorescence Correlation Spectroscopy (iFCS) is a variant of FCS where unlabeled particles in solution, or domains in membranes, displace their surrounding, signal-generating molecules and thereby generate fluctuations. iFCS has to date been applied to unlabeled as well as labeled particles and protein molecules, using fluorescence as well as Raman scattering as a signal source, in diffraction-limited detection volumes as well as in nano-wells, and on fixed surfaces as well as in lipid bilayers. This review describes these applications and discusses the potentials and pitfalls when using iFCS.

摘要

反向荧光相关光谱（iFCS）是一种 FCS 的变体，其中溶液中的未标记粒子或膜中的域会置换其周围的信号产生分子，从而产生波动。迄今为止，iFCS 已应用于未标记和标记的粒子以及蛋白质分子，使用荧光和拉曼散射作为信号源，在衍射受限的检测体积以及纳米井中，在固定表面以及脂质双层上。本综述描述了这些应用，并讨论了使用 iFCS 时的潜力和陷阱。