Ning Ying-Jun, Lu Xin-Jiang, Chen Jiong
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, 315211, China.
Dev Comp Immunol. 2018 Jul;84:37-47. doi: 10.1016/j.dci.2018.02.002. Epub 2018 Feb 9.
Tissue factor (TF) plays an important role in the host's immune system as the principal initiator of coagulation. However, the precise function of TF in teleosts remains unclear. We determined the cDNA sequence of TF from ayu Plecoglossus altivelis (PaTF). The PaTF transcript was expressed in all tested tissues, and changes in expression were observed in tissues and monocytes/macrophages (MO/MФ) upon infection with Vibrio anguillarum. PaTF was prokaryotically expressed and purified to prepare anti-PaTF antibodies. Western blot analysis revealed that native PaTF was glycosylated in thrombocytes, but not in ayu MO/MФ. Microparticles could transfer PaTF to thrombocytes. PaTF neutralization or knockdown led to anti-inflammatory status in ayu MO/MФ upon V. anguillarum infection. PaTF neutralization reduced the apoptosis of ayu MO/MФ and improve survival rate in V. anguillarum-infected ayu. Our results indicate that PaTF plays a role in ayu immune response against bacterial infection as a pro-inflammatory mediator.
组织因子（TF）作为凝血的主要启动因子，在宿主免疫系统中发挥着重要作用。然而，TF在硬骨鱼中的具体功能仍不清楚。我们测定了香鱼（Plecoglossus altivelis）TF（PaTF）的cDNA序列。PaTF转录本在所有测试组织中均有表达，在用鳗弧菌感染后，在组织以及单核细胞/巨噬细胞（MO/MФ）中观察到了表达变化。对PaTF进行原核表达和纯化以制备抗PaTF抗体。蛋白质免疫印迹分析显示，天然PaTF在血小板中发生了糖基化，但在香鱼MO/MФ中未发生糖基化。微粒可将PaTF转移至血小板。在鳗弧菌感染后，中和或敲低PaTF会使香鱼MO/MФ处于抗炎状态。中和PaTF可减少香鱼MO/MФ的凋亡，并提高鳗弧菌感染的香鱼的存活率。我们的结果表明，PaTF作为一种促炎介质，在香鱼针对细菌感染的免疫反应中发挥作用。
