Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
Department of Food Science and Biotechnology, Kunsan National University, Kunsan 573-701, Republic of Korea.
Int J Biol Macromol. 2018 Jun;112:483-489. doi: 10.1016/j.ijbiomac.2018.01.212. Epub 2018 Feb 2.
Polysaccharides were extracted by Celluclast-assisted hydrolysis and ethanol precipitation from Hizikia fusiforme (HFPS), and their in vitro antioxidant effects were evaluated in Vero cells. HFPS contains 63.56±0.32% sulfate polysaccharides, which comprise fucose (53.53%), galactose (23.15%), glucose (5.95%), and xylose (17.37%). HFPS scavenged DPPH, alkyl, and hydroxyl radicals at IC values of 0.81±0.02, 0.25±0.02, and 0.21±0.03mg/mL, respectively. HFPS significantly reduced HO-induced cytotoxicity in Vero cells. Furthermore, HFPS reduced intracellular ROS levels, and HO-induced apoptosis. HFPS has a strong protective effect against HO-stimulated oxidative stress in vivo in zebrafish, and showed improved survival rate, decreased heart rate, and reduced ROS generation and cell death. These results suggest that HFPS may be useful as an antioxidant in medical and cosmetic industries.
多糖用纤维素酶辅助水解和乙醇沉淀从裙带菜（HFPS）中提取，并在 Vero 细胞中评估其体外抗氧化作用。HFPS 含有 63.56±0.32%的硫酸多糖，由岩藻糖（53.53%）、半乳糖（23.15%）、葡萄糖（5.95%）和木糖（17.37%）组成。HFPS 在 IC 值为 0.81±0.02、0.25±0.02 和 0.21±0.03mg/mL 时，分别清除 DPPH、烷基和羟基自由基。HFPS 显著降低了 Vero 细胞中 HO 诱导的细胞毒性。此外，HFPS 降低了细胞内 ROS 水平和 HO 诱导的细胞凋亡。HFPS 对斑马鱼 HO 刺激的氧化应激具有很强的保护作用，表现为存活率提高、心率降低、ROS 生成和细胞死亡减少。这些结果表明，HFPS 可能在医药和化妆品行业作为抗氧化剂使用。
