Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
Int J Biol Macromol. 2016 Oct;91:804-11. doi: 10.1016/j.ijbiomac.2016.05.111. Epub 2016 Jun 11.
The aim of this study was to analyze antioxidant properties of a polysaccharide isolated from Psidium guajava leaves (PS-PGL) in vitro including its radical scavenging activities and protective effects against damage to cells as well as in vivo in zebrafish. The water extract of P. guajava leaves (WE-PGL) and PS-PGL showed strong radical scavenging effects in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical. Compared to WE-PGL, PS-PGL enhanced all scavenging activities and in particular strongly scavenged the hydroxyl radical (50% inhibitory concentration [IC50], 0.02mg/mL). In addition, PS-PGL exerted a protective effect against hydrogen peroxide-induced oxidative stress and against toxicity to Vero cells. Furthermore, in vivo experiments using zebrafish embryos indicated that treatment with hydrogen peroxide decreased the survival rate and heart-beating rate of zebrafish embryos, whereas these problems were reduced by PS-PGL treatment. Moreover, PS-PGL inhibited hydrogen peroxide-induced reactive oxygen species (ROS) production, lipid peroxidation, and cell death. Taken together, these results suggest that PS-PGL may be useful as a beneficial antioxidant material in the food and cosmetic industries.
本研究旨在分析番石榴叶中分离得到的多糖（PS-PGL）的体外抗氧化特性，包括其自由基清除活性以及对细胞损伤的保护作用，同时也在斑马鱼体内进行了研究。番石榴叶水提物（WE-PGL）和 PS-PGL 均表现出较强的 2,2-二苯基-1-苦基肼（DPPH）、羟基和烷基自由基清除活性。与 WE-PGL 相比，PS-PGL 增强了所有的清除活性，特别是对羟基自由基（半抑制浓度 [IC50]，0.02mg/mL）的清除能力更强。此外，PS-PGL 对过氧化氢诱导的氧化应激和 Vero 细胞毒性具有保护作用。此外，利用斑马鱼胚胎进行的体内实验表明，过氧化氢处理降低了斑马鱼胚胎的存活率和心跳率，而 PS-PGL 处理则降低了这些问题的发生。此外，PS-PGL 抑制了过氧化氢诱导的活性氧（ROS）产生、脂质过氧化和细胞死亡。综上所述，这些结果表明 PS-PGL 可能作为一种有益的抗氧化物质在食品和化妆品行业中具有应用潜力。
