Velayutham Manikandan, Sarkar Purabi, Karuppiah Kanchana M, Arumugam Priyadharsan, Shajahan Shanavas, Abu Haija Mohammad, Ahamad Tansir, Arasu Mariadhas Valan, Al-Dhabi Naif Abdullah, Choi Ki-Choon, Guru Ajay, Arockiaraj Jesu
Department of Medical Biotechnology and Integrative Physiology, Institute of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai 602105, Tamil Nadu, India.
Department of Molecular Medicine, School of Allied Healthcare and Sciences, Jain Deemed-to-be University, Whitefield, Bangalore 560066, Karnataka, India.
ACS Omega. 2023 May 17;8(21):18543-18553. doi: 10.1021/acsomega.3c00336. eCollection 2023 May 30.
One of the most common diseases in women is breast cancer, which has the highest death globally. Surgery, chemotherapy, hormone treatments, and radiation are the current treatment options for breast cancer. However, these options have several adverse side effects. Recently, peptide-based drugs have gained attention as anticancer therapy. Studies report that peptides from biological toxins such as venom and virulent pathogenic molecules have potential therapeutic effects against multiple diseases, including cancers. This study reports on the anticancer effect of a short peptide, PS9, derived from a virulent protein, glycosyl hydrolase, of an aquatic fungus, . This peptide arrests MCF-7 proliferation by regulating intercellular reactive oxygen species (ROS) and apoptotic pathways. Based on the potential for the anticancer effect of PS9, from the analysis, analyses using MCF-7 cells were executed. PS9 showed a dose-dependent activity; its IC value was 25.27-43.28 μM at 24 h. The acridine orange/ethidium bromide (AO/EtBr) staining, to establish the status of apoptosis in MCF-7 cells, showed morphologies for early and late apoptosis and necrotic cell death. The 2,7-dichlorodihydrofluorescein diacetate (DCFDA) staining and biochemical analyses showed a significant increase in reactive oxygen species (ROS). Besides, PS9 has been shown to regulate the caspase-mediated apoptotic pathway. PS9 is nontoxic, , and zebrafish larvae. Together, PS9 may have an anticancer effect .
女性最常见的疾病之一是乳腺癌，它在全球范围内的死亡率最高。手术、化疗、激素治疗和放疗是目前乳腺癌的治疗选择。然而，这些选择有若干不良副作用。最近，基于肽的药物作为抗癌疗法受到关注。研究报告称，来自毒液和有毒致病分子等生物毒素的肽对包括癌症在内的多种疾病具有潜在治疗作用。本研究报告了一种源自水生真菌的有毒蛋白糖基水解酶的短肽PS9的抗癌作用。该肽通过调节细胞间活性氧（ROS）和凋亡途径来抑制MCF-7细胞增殖。基于PS9的抗癌作用潜力，进行了来自分析，使用MCF-7细胞的分析。PS9表现出剂量依赖性活性；其在24小时时的IC值为25.27 - 43.28μM。吖啶橙/溴化乙锭（AO/EtBr）染色用于确定MCF-7细胞中的凋亡状态，显示出早期和晚期凋亡以及坏死细胞死亡的形态。2,7 - 二氯二氢荧光素二乙酸酯（DCFDA）染色和生化分析表明活性氧（ROS）显著增加。此外，PS9已被证明可调节半胱天冬酶介导的凋亡途径。PS9对，和斑马鱼幼虫无毒。总之，PS9可能具有抗癌作用。
