Young D A, Chi M M, Lowry O H
Am J Physiol. 1986 Jun;250(6 Pt 1):C813-20. doi: 10.1152/ajpcell.1986.250.6.C813.
This study was made to test the validity of a simple biopsy technique for assessing the metabolic capacity of skeletal muscle. The biopsy is stimulated under mineral oil without attachment, i.e., without load or tension, then freeze-clamped and assayed for ATP, phosphocreatine, glucose 6-phosphate, and lactate. The mineral oil creates a closed anaerobic system. Background studies demonstrated in the absence of a load, metabolic changes with stimulation were little affected by cutting the fibers to obtain the biopsy; and high-energy phosphate (approximately P) consumption during a brief tetanus was not much lower than that for an isometric tetanus. Individual fast-twitch oxidative-glycolytic (IIA) and fast-twitch glycolytic (IIB) fibers obtained from the freeze-clamped biopsy showed distinct differences in approximately P consumption and metabolic changes. The results indicate that this technique could be useful for studies of normal and pathological human muscle.
本研究旨在测试一种用于评估骨骼肌代谢能力的简单活检技术的有效性。在矿物油下对活检组织进行无附着刺激，即无负荷或张力，然后进行冷冻钳夹，并测定三磷酸腺苷（ATP）、磷酸肌酸、6-磷酸葡萄糖和乳酸。矿物油形成一个封闭的厌氧系统。背景研究表明，在无负荷的情况下，获取活检组织时切断纤维对刺激引起的代谢变化影响很小；短暂强直收缩期间高能磷酸（约P）的消耗量与等长强直收缩相比降低不多。从冷冻钳夹活检组织中获取的单个快肌氧化糖酵解型（IIA）和快肌糖酵解型（IIB）纤维在约P消耗量和代谢变化方面表现出明显差异。结果表明，该技术可能对正常和病理状态下的人体肌肉研究有用。
