Pharmaceutical Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Quality Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Environ Toxicol Pharmacol. 2018 Mar;58:180-188. doi: 10.1016/j.etap.2018.01.006. Epub 2018 Jan 16.
Sulfur mustard is an alkylating agent which cause to short and long term incapacitations on various organs including lung. There is no definite treatment for lung disorders induced by SM exposure. In the present study, the preventive effect of Zataria multiflora (Z. multiflora) on hematological parameters, oxidant/antioxidant markers and pulmonary function tests (PFT) in veterans, 27-30 years after exposed to SM were studied.
Forty seven veterans allocated to three groups included: placebo group (P) and two groups treated with 5 and 10 mg/kg/day of Z. multiflora (Zat 5 and Zat 10). Drugs were prescribed in a double-blind manner for two months. Total and different WBC, hematological indices, oxidant/antioxidant markers and PFT values included; force vital capacity (FVC) and peak expiratory flow (PEF) were assessed at the beginning (step 0), one and two month (step I and II, respectively) after starting treatment.
Total and different white blood cell in Zat 5 and 10 mg/kg treated groups in Step I and II were significantly decreased compared to Step 0 (p < 0.05 to p < 0.001). The levels of thiol, superoxide dismutase (SOD) and catalase (CAT) in Zat 5 and 10 mg/kg treated groups in step I and II were significantly increased (p < 0.05 to p < 0.001) but the level of malondialdehyde (MDA) significantly decreased in two treatment groups compared to Step 0 (p < 0.05 and p < 0.001 respectively). FVC and PEF values were significant increase in Zat 5 and 10 mg/kg treated groups in step I and II compared to step 0 (p < 0.05 to p < 0.001). Furthermore, FVC and PEF values in Zat 5 mg/kg were also increased in step II compared to step I (p < 0.01 for both). The percentage improvement of total and differential WBC, oxidant/antioxidant markers, FVC and PEF values during two moth treatment period significantly improved in the treated groups compared to the placebo group.
Z. multiflora reduces inflammatory cells and oxidant biomarkers, while increase antioxidant biomarkers and improved PFT tests in SM exposed patients in a two moth treatment period.
芥子气是一种烷化剂，可导致肺部等各种器官的短期和长期失能。目前尚无针对 SM 暴露引起的肺部疾病的明确治疗方法。在本研究中，研究了香薷（Zataria multiflora，Z. multiflora）对 27-30 年后暴露于 SM 的退伍军人的血液学参数、氧化应激/抗氧化标志物和肺功能测试（PFT）的预防作用。
将 47 名退伍军人分为三组：安慰剂组（P）和两组分别用 5 和 10mg/kg/天的 Z. multiflora（Zat 5 和 Zat 10）治疗。药物以双盲方式开处方，持续两个月。总白细胞和不同白细胞、血液学指标、氧化应激/抗氧化标志物和 PFT 值包括：用力肺活量（FVC）和呼气峰值流量（PEF）在开始治疗前（步骤 0）、治疗开始后 1 个月和 2 个月（分别为步骤 I 和 II）进行评估。
Zat 5 和 10mg/kg 治疗组在步骤 I 和 II 时的总白细胞和不同白细胞均明显低于步骤 0（p<0.05 至 p<0.001）。Zat 5 和 10mg/kg 治疗组在步骤 I 和 II 时的巯基、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平显著升高（p<0.05 至 p<0.001），但与步骤 0 相比，两种治疗组的丙二醛（MDA）水平均显著降低（分别为 p<0.05 和 p<0.001）。Zat 5 和 10mg/kg 治疗组在步骤 I 和 II 时的 FVC 和 PEF 值均明显高于步骤 0（p<0.05 至 p<0.001）。此外，Zat 5mg/kg 组在步骤 II 时的 FVC 和 PEF 值也高于步骤 I（均为 p<0.01）。与安慰剂组相比，在两个月的治疗期间，总白细胞和不同白细胞、氧化应激/抗氧化标志物、FVC 和 PEF 值的改善百分比在治疗组中显著提高。
在两个月的治疗期间，香薷可减少 SM 暴露患者的炎症细胞和氧化应激生物标志物，同时增加抗氧化生物标志物并改善 PFT 测试。
