Tucker S B, Flannigan S A, Dunbar M, Drotman R B
Arch Dermatol. 1986 Jun;122(6):660-5.
The rabbit ear comedogenicity assay is useful as a screening procedure for evaluating agents that come in contact with human skin. Controversy exists regarding the reliability of this assay because of differences in results from various laboratories. The subjective nature of the standard method of grading may also contribute to this variation. We use a more objective comedogenicity assay that utilizes increasing follicular orifice size on the rabbit ear as a measure of comedogenic activity. A generally linear increase in the degree of follicular orifice area was noted with several agents evaluated over a four-week application period. Further, a noninvasive Silastic elastomer mold was used to allow measurement of the same follicular orifice areas over time.
兔耳致粉刺性试验作为一种筛选程序，可用于评估接触人体皮肤的制剂。由于各实验室结果存在差异，该试验的可靠性存在争议。分级标准方法的主观性也可能导致这种差异。我们采用了一种更客观的致粉刺性试验，该试验利用兔耳上逐渐增大的毛囊口大小作为致粉刺活性的指标。在四周的应用期内，对几种制剂进行评估时，发现毛囊口面积的程度总体呈线性增加。此外，使用了一种非侵入性的硅橡胶弹性体模具，以便能够随时间测量相同的毛囊口面积。
