Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa.
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa.
Fungal Genet Biol. 2018 Apr;113:32-41. doi: 10.1016/j.fgb.2018.01.007. Epub 2018 Jan 31.
Sexual reproduction in the Ascomycota is controlled by genes encoded at the mating-type or MAT1 locus. The two allelic versions of this locus in heterothallic species, referred to as idiomorphs, are defined by the MAT1-1-1 (for the MAT1-1 idiomorph) and MAT1-2-1 (for the MAT1-2 idiomorph) genes. Both idiomorphs can contain additional genes, although the contents of each is typically specific to and conserved within particular Pezizomycotina lineages. Using full genome sequences, complemented with conventional PCR and Sanger sequencing, we compared the mating-type idiomorphs in heterothallic species of Thielaviopsis (Ceratocystidaceae). The analyses showed that the MAT1-1 idiomorph of T. punctulata, T. paradoxa, T. euricoi, T. ethacetica and T. musarum harboured only the expected MAT1-1-1 gene. In contrast, the MAT1-2 idiomorph of T. punctulata, T. paradoxa and T. euricoi encoded the MAT1-2-1, MAT1-2-7 and MAT1-1-2 genes. Of these, MAT1-2-1 and MAT1-2-7 are genes previously reported in this idiomorph, while MAT1-1-2 is known only in the MAT1-1 idiomorph. Phylogenetic analysis showed that the Thielaviopsis MAT1-1-2 groups with the known homologues of this gene in other Microascales, thus confirming its annotation. Previous work suggests that MAT1-1-2 is involved in fruiting body development, a role that would be unaffected by its idiomorphic position. This notion is supported by our findings for the MAT1 locus structure in Thielaviopsis species. This also serves as the first example of a MAT1-1-specific gene restricted to only the MAT1-2 idiomorph.
子囊菌的有性生殖受位于交配型或 MAT1 基因座的基因控制。在异宗配合的物种中，这个基因座的两个等位基因版本，称为异型，由 MAT1-1-1（MAT1-1 异型）和 MAT1-2-1（MAT1-2 异型）基因定义。这两个异型都可以包含其他基因，尽管每个异型的内容通常是特定的，并在特定的 Pezizomycotina 谱系中保守。使用全基因组序列，并辅以常规 PCR 和 Sanger 测序，我们比较了异宗配合的 Thielaviopsis（Ceratocystidaceae）物种的交配型异型。分析表明，T. punctulata、T. paradoxa、T. euricoi、T. ethacetica 和 T. musarum 的 MAT1-1 异型仅含有预期的 MAT1-1-1 基因。相比之下，T. punctulata、T. paradoxa 和 T. euricoi 的 MAT1-2 异型编码了 MAT1-2-1、MAT1-2-7 和 MAT1-1-2 基因。其中，MAT1-2-1 和 MAT1-2-7 是以前在这个异型中报道过的基因，而 MAT1-1-2 只在 MAT1-1 异型中被发现。系统发育分析表明，Thielaviopsis MAT1-1-2 与其他 Microascales 中该基因的已知同源物聚在一起，从而证实了其注释。先前的工作表明，MAT1-1-2 参与了子实体的发育，其异型位置不会影响其功能。这一观点得到了我们在 Thielaviopsis 物种中发现的 MAT1 基因座结构的支持。这也是第一个仅限于 MAT1-2 异型的 MAT1-1 特异性基因的例子。
