School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, PR China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, PR China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
Int J Biol Macromol. 2018 Jun;112:433-441. doi: 10.1016/j.ijbiomac.2018.01.204. Epub 2018 Feb 1.
Osteoporosis is a frequent disease among the elderly especially in postmenopausal women. Achyranthes bidentata is a traditional Chinese medicine used to strengthen bones. Here, A. bidentata polysaccharides (ABPs) were confirmed to have anti-osteoporosis effects. This study discovered biomarkers by comparing normal and osteoporosis rats and evaluated the effects of ABPs on osteoporosis based on the UPLC/Q-TOF-MS-based metabolomics analysis. We could then predict the underlying mechanisms from the perspective of metabolomics. Osteoporotic rats were treated with ABPs, and serum was then sampled for metabolic analysis. Glutarylcarnitine, lysoPC (18:1) and 9-cis-retinoic acid were identified as biomarkers. The ABPs could significantly increase these biomarkers, and this indicated that ABPs curing osteoporosis regulated lipid metabolism. The UPLC/Q-TOF-MS-based metabolomics analysis offered a potential strategy to evaluate the anti-osteoporosis effects of ABPs and to explain the relative mechanisms. Furthermore, the ABPs have good potential for treating osteoporosis.
骨质疏松症是老年人，特别是绝经后妇女的常见病。牛膝是一种传统的中药，用于强筋健骨。本研究证实牛膝多糖（ABPs）具有抗骨质疏松作用。通过比较正常和骨质疏松大鼠，本研究利用基于 UPLC/Q-TOF-MS 的代谢组学分析，发现了生物标志物，并评估了 ABPs 对骨质疏松症的影响。然后，我们可以从代谢组学的角度预测潜在的机制。用 ABPs 处理骨质疏松大鼠，然后采集血清进行代谢分析。鉴定出谷氨酰肉碱、溶血磷脂酰胆碱（18:1）和 9-顺式视黄酸为生物标志物。ABPs 能显著增加这些生物标志物，这表明 ABPs 治疗骨质疏松症可调节脂代谢。基于 UPLC/Q-TOF-MS 的代谢组学分析为评估 ABPs 的抗骨质疏松作用和解释相关机制提供了一种潜在的策略。此外，ABPs 具有治疗骨质疏松症的良好潜力。
