外泌体长链非编码RNA 91H通过调节HNRNPK的表达与结直肠癌的不良发展相关。

Exosomal lncRNA 91H is associated with poor development in colorectal cancer by modifying HNRNPK expression.

作者信息

Gao Tianyi, Liu Xiangxiang, He Bangshun, Nie Zhenlin, Zhu Chengbin, Zhang Pei, Wang Shukui

机构信息

1Department of Clinical Laboratory, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006 Jiangsu China.

2Central Laboratory, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210006 Jiangsu China.

出版信息

Cancer Cell Int. 2018 Jan 23;18:11. doi: 10.1186/s12935-018-0506-2. eCollection 2018.

BACKGROUND

Exosomes mediated transfer of lncRNA 91H may play a critical role in the development of CRC. However, few studies have proved the mechanism. So we performed this study to deeply explore the biological functions of exosomal 91H in the development and progression of CRC.

METHODS

The association between lncRNA 91H and exosomes was detected in vitro and vivo. Then RNA pulldown and RIP were used to detect how lncRNA 91H affect CRC IGF2 express. At last, clinic pathological significance of exosomal 91H was evaluated by Cox proportional hazards model.

RESULTS

We found that serum lncRNA 91H expression was closely related to cancer exosomes in vitro and vivo which may enhance tumor-cell migration and invasion in tumor development by modifying HNRNPK expression. Then the clinic pathological significance of exosomal 91H was evaluated which demonstrated that CRC patients with high lncRNA 91H expression usually showed a higher risk in tumor recurrence and metastasis than patients with low lncRNA 91H expression (P < 0.05).

CONCLUSION

All these data suggested that exosomal lncRNA 91H enhancing CRC metastasis by modifying HNRNPK expression might be an early plasma-based biomarker for CRC recurrence or metastasis. Further large-scale studies are needed to confirm our findings.

背景

外泌体介导的长链非编码RNA 91H的转移可能在结直肠癌的发展中起关键作用。然而，很少有研究证实其机制。因此，我们进行了这项研究，以深入探讨外泌体91H在结直肠癌发生发展中的生物学功能。

方法

在体外和体内检测长链非编码RNA 91H与外泌体之间的关联。然后使用RNA下拉和RNA免疫沉淀技术检测长链非编码RNA 91H如何影响结直肠癌中胰岛素样生长因子2（IGF2）的表达。最后，通过Cox比例风险模型评估外泌体91H的临床病理意义。

结果

我们发现，血清长链非编码RNA 91H的表达在体外和体内均与癌外泌体密切相关，其可能通过改变异质性核糖核蛋白K（HNRNPK）的表达，在肿瘤发展过程中增强肿瘤细胞的迁移和侵袭能力。随后评估了外泌体91H的临床病理意义，结果表明，长链非编码RNA 91H高表达的结直肠癌患者通常比长链非编码RNA 91H低表达的患者具有更高的肿瘤复发和转移风险（P<0.05）。