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一种用于对癌症患者和小鼠肿瘤模型的游离DNA进行全基因组亚硫酸氢盐测序的方法的开发。

Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model.

作者信息

Maggi Elaine C, Gravina Silvia, Cheng Haiying, Piperdi Bilal, Yuan Ziqiang, Dong Xiao, Libutti Steven K, Vijg Jan, Montagna Cristina

机构信息

Department of Genetics, Albert Einstein College of Medicine, New York, NY, United States.

Department of Oncology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY, United States.

出版信息

Front Genet. 2018 Jan 23;9:6. doi: 10.3389/fgene.2018.00006. eCollection 2018.

DOI:10.3389/fgene.2018.00006
PMID:29410677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5787102/
Abstract

The goal of this study was to develop a method for whole genome cell-free DNA (cfDNA) methylation analysis in humans and mice with the ultimate goal to facilitate the identification of tumor derived DNA methylation changes in the blood. Plasma or serum from patients with pancreatic neuroendocrine tumors or lung cancer, and plasma from a murine model of pancreatic adenocarcinoma was used to develop a protocol for cfDNA isolation, library preparation and whole-genome bisulfite sequencing of ultra low quantities of cfDNA, including tumor-specific DNA. The protocol developed produced high quality libraries consistently generating a conversion rate >98% that will be applicable for the analysis of human and mouse plasma or serum to detect tumor-derived changes in DNA methylation.

摘要

本研究的目标是开发一种用于人类和小鼠全基因组游离DNA（cfDNA）甲基化分析的方法，最终目标是便于识别血液中肿瘤衍生的DNA甲基化变化。使用来自胰腺神经内分泌肿瘤或肺癌患者的血浆或血清，以及来自胰腺腺癌小鼠模型的血浆，开发了一种用于超低量cfDNA（包括肿瘤特异性DNA）分离、文库制备和全基因组亚硫酸氢盐测序的方案。所开发的方案产生了高质量的文库，转化率始终>98%，适用于分析人类和小鼠的血浆或血清，以检测DNA甲基化中肿瘤衍生的变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/a6c79f6b248b/fgene-09-00006-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/afb33601f449/fgene-09-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/031db1266e33/fgene-09-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/156c515ff29a/fgene-09-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/c8827a44bd84/fgene-09-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/d0bbffc7bff9/fgene-09-00006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/20ef1737dfe6/fgene-09-00006-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/a6c79f6b248b/fgene-09-00006-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/afb33601f449/fgene-09-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/031db1266e33/fgene-09-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/156c515ff29a/fgene-09-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/c8827a44bd84/fgene-09-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/d0bbffc7bff9/fgene-09-00006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/20ef1737dfe6/fgene-09-00006-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5113/5787102/a6c79f6b248b/fgene-09-00006-g007.jpg

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