Solari A, Zourgui L, Carvallo P, Tarrago-Litvak L
Biol Cell. 1986;56(1):23-9. doi: 10.1111/j.1768-322x.1986.tb00439.x.
The localization of DNA polymerases in Xenopus laevis oocytes and eggs was studied. Oocytes have a high level of DNA polymerase alpha activity detected exclusively in the nuclei, while mitochondria contain all the DNA polymerase activity of the gamma type. No DNA polymerase beta activity is present in the nuclear fraction. Moreover, the beta activity is not present in unfertilized eggs. In oocytes, DNA polymerase alpha is weakly bound to the nucleoplasm. The leakage of the enzyme from whole nuclei can be prevented using polyvinylpyrrolidone, a nuclear pore sealing agent.
对非洲爪蟾卵母细胞和卵中DNA聚合酶的定位进行了研究。卵母细胞具有高水平的DNA聚合酶α活性，仅在细胞核中检测到，而线粒体含有所有γ型DNA聚合酶活性。核部分中不存在DNA聚合酶β活性。此外，未受精卵中也不存在β活性。在卵母细胞中，DNA聚合酶α与核质结合较弱。使用核孔封闭剂聚乙烯吡咯烷酮可以防止该酶从整个细胞核中泄漏。
