感染猿猴病毒40的生长细胞中的DNA聚合酶活性
DNA polymerase activities in growing cells infected with simian virus 40.
作者信息
Méchali M, Girard M, de Recondo A M
出版信息
J Virol. 1977 Jul;23(1):117-25. doi: 10.1128/JVI.23.1.117-125.1977.
Abstract
Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity of DNA polymerase-alpha increased five- to sixfold in the nuclear Triton wash and threefold in the nuclei and then remained enhanced only inside the nuclei. That of DNA polymerases-beta and gamma increased mostly in the nuclei after infection. These results suggest that DNA polymerase-alpha could be the major enzyme involved in SV40 DNA replication.
摘要
用猿猴病毒40（SV40）感染生长中的CV1细胞，并对细胞质、核Triton洗涤液和细胞核中的DNA聚合酶α、β和γ水平进行分析。在细胞质部分，SV40感染后α-、β-或γ-聚合酶的量保持不变。DNA聚合酶α的活性在核Triton洗涤液中增加了五到六倍，在细胞核中增加了三倍，然后仅在细胞核内保持增强。DNA聚合酶β和γ的活性在感染后主要在细胞核中增加。这些结果表明DNA聚合酶α可能是参与SV40 DNA复制的主要酶。
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