Maraldi N M, Marinelli F, Galanzi A, Zini N, Ognibene A, Del Coco R, Matteucci A, Papa S
Biol Cell. 1986;56(1):31-9. doi: 10.1111/j.1768-322x.1986.tb00440.x.
The morphology of intact or membrane-deprived interphase nuclei has been analysed by freeze-fracture electron microscopy. This method appears particularly useful for providing information on the distribution and organisation of chromatin and ribonucleoproteins in the absence of dehydration and embedding artifacts of conventional electron microscope techniques which, among other effects, appear to affect heterochromatin distribution, inducing its aggregation along the nuclear envelope. The main levels of chromatin superstructure, from nucleosome to solenoid fibres, are detectable in the replicas of freeze-fractured nuclei on the basis of the size of their shadow, a parameter particularly suitable for automated image analyses.
通过冷冻断裂电子显微镜分析了完整的或去除膜的间期细胞核的形态。这种方法对于在不存在传统电子显微镜技术的脱水和包埋假象的情况下提供关于染色质和核糖核蛋白的分布及组织的信息似乎特别有用，传统电子显微镜技术除了其他影响外，似乎会影响异染色质分布，导致其沿核膜聚集。基于其阴影大小，在冷冻断裂细胞核的复制品中可检测到从核小体到螺线管纤维的染色质超结构的主要层次，阴影大小是一个特别适合自动图像分析的参数。
