Maraldi N M, Marinelli F, Cocco L, Papa S, Santi P, Manzoli F A
Exp Cell Res. 1986 Apr;163(2):349-62. doi: 10.1016/0014-4827(86)90066-2.
The ultrastructural organization of nuclear matrix, purified from intact or membrane-denuded rat liver nuclei, has been analysed by means of freeze-fracturing technique. This method avoids dehydration and embedding which, in conventional thin sectioning, partly distort or mask the matrix ultrastructure. The various matrix components, and mainly the peripheral lamina and the inner network revealed complex arrangements undetectable with conventional techniques. Morphometric analyses performed with a Texture Analysis System (TAS) Leitz, allowed to obtain precise information on the matrix constituents, based on the histograms of their size distribution. These textural characteristics have been utilized in order to identify, by means of a particular computer programme, the putative matrix localization within intact freeze-fractured nuclei.
利用冷冻断裂技术分析了从完整的或去除膜的大鼠肝细胞核中纯化得到的核基质的超微结构组织。该方法避免了脱水和包埋，而在传统的超薄切片中，脱水和包埋会部分扭曲或掩盖基质的超微结构。各种基质成分，主要是外周板层和内部网络，呈现出用传统技术无法检测到的复杂排列。使用徕卡纹理分析系统（TAS）进行的形态计量分析，基于基质成分大小分布的直方图，能够获得关于它们的精确信息。这些纹理特征已被用于通过一个特定的计算机程序来识别完整的冷冻断裂核内假定的基质定位。
