Institute of Agricultural Facilities and Equipment, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, Jiangsu, 210014, China.
Key Laboratory for Protected Agricultural Engineering in the Middle and Lower Reaches of Yangtze River, Ministry of Agriculture, 50 Zhongling Street, Nanjing, Jiangsu, 210014, China.
Anal Bioanal Chem. 2018 Mar;410(8):2253-2262. doi: 10.1007/s00216-018-0895-z. Epub 2018 Feb 6.
Mycotoxins produced by different species of fungi may coexist in cereals and feedstuffs, and could be highly toxic for humans and animals. For quantification of multiple mycotoxins in cereals, we developed a paper-based mycotoxin immune-affinity array. First, paper-based microzone arrays were fabricated by photolithography. Then, monoclonal mycotoxin antibodies were added in a copolymerization reaction with a cross-linker to form an immune-affinity monolith on the paper-based microzone array. With use of a competitive immune-response format, paper-based mycotoxin immune-affinity arrays were successfully applied to detect mycotoxins in samples. The detection limits for deoxynivalenol, zearalenone, T-2 toxin, and HT-2 toxin were 62.7, 10.8, 0.36, and 0.23 μg·kg, respectively, which meet relevant requirements for these compounds in food. The recovery rates were 81-86% for deoxynivalenol, 89-117% for zearalenone, 79-86% for T-2 toxin, and 78-83% for HT-2 toxin, and showed the paper-based immune-affinity arrays had good reproducibility. In summary, the paper-based mycotoxin immune-affinity array provides a sensitive, rapid, accurate, stable, and convenient platform for detection of multiple mycotoxins in agro-foods. Graphical abstract Paper-based immune-affinity monolithic array. DON deoxynivalenol, HT-2 HT-2 toxin, T-2 T-2 toxin, PEGDA polyethylene glycol diacrylate, ZEN zearalenone.
真菌产生的不同种霉菌毒素可能共存于谷物和饲料中，对人类和动物可能具有高毒性。为了定量检测谷物中的多种霉菌毒素，我们开发了一种基于纸张的霉菌毒素免疫亲和阵列。首先，通过光刻法制造基于纸张的微区阵列。然后，将单克隆霉菌毒素抗体与交联剂共聚反应添加到微区阵列的基于纸张的免疫亲和单柱中。使用竞争免疫反应的格式，成功地将基于纸张的霉菌毒素免疫亲和阵列应用于检测样品中的霉菌毒素。脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、T-2 毒素和 HT-2 毒素的检测限分别为 62.7、10.8、0.36 和 0.23μg·kg，符合食品中这些化合物的相关要求。脱氧雪腐镰刀菌烯醇的回收率为 81-86%，玉米赤霉烯酮的回收率为 89-117%，T-2 毒素的回收率为 79-86%，HT-2 毒素的回收率为 78-83%，表明基于纸张的免疫亲和阵列具有良好的重现性。总之，基于纸张的霉菌毒素免疫亲和阵列为检测农产品中的多种霉菌毒素提供了一个灵敏、快速、准确、稳定和方便的平台。
