Allen B G, Bridges M, Roufogalis B D, Katz S
Cell Calcium. 1986 Jun;7(3):161-8. doi: 10.1016/0143-4160(86)90019-9.
The (Ca2+ + Mg2+)-ATPase present per mg of protein in erythrocyte membranes of controls and patients with cystic fibrosis (CF) was determined by estimation of the levels of its phosphoprotein. In the presence of 10 mM free Ca2+, which inhibits phosphoprotein decomposition, significantly less phosphoprotein intermediate, ECaP, was found in erythrocyte membranes from CF patients than in age- and sex-matched controls; this correlated with a significant decrease in (Ca2+ + Mg2+)-ATPase activity. These observations indicate a decrease in the number of functional (Ca2+ + Mg2+)-ATPase molecules in erythrocyte membranes from CF patients or an alteration in either the structure of the pump protein or the composition of its environment.
通过对磷蛋白水平的评估，测定了对照者和囊性纤维化（CF）患者红细胞膜中每毫克蛋白质所含的（Ca2+ + Mg2+）-ATP酶。在存在10 mM游离Ca2+（可抑制磷蛋白分解）的情况下，CF患者红细胞膜中发现的磷蛋白中间体ECaP明显少于年龄和性别匹配的对照者；这与（Ca2+ + Mg2+）-ATP酶活性的显著降低相关。这些观察结果表明，CF患者红细胞膜中功能性（Ca2+ + Mg2+）-ATP酶分子数量减少，或者泵蛋白的结构或其周围环境的组成发生了改变。
