Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
University of Chinese Academy of Sciences , Beijing 100190, China.
ACS Appl Mater Interfaces. 2018 Feb 28;10(8):6956-6964. doi: 10.1021/acsami.7b19010. Epub 2018 Feb 19.
Large segmental bone defect repairing remains a big challenge in clinics, and synthetic bone grafts suitable for this purpose are still highly demanded. In this article, hydrophilic composite scaffolds (bioactive hollow particle (BHP)-gel scaffold) composed of bioactive hollow nanoparticles and cross-linked gelatin have been developed. The bioactive nanoparticles have a porous structure as well as high specific surface area; thus, they interact strongly with gelatin to overcome the swelling problem that a hydrophilic polymer scaffold will usually face. With this combination, these BHP-gel scaffolds showed porous structure and mechanical properties similar to those of the cancellous bone. They also showed excellent bioactivity and cell growth promotion performance in vitro. The best of them, namely, 10BHP-gel scaffold, was evaluated in vivo on a rat femur model, where it was found that the 5 mm segmental bone defect almost healed with new bone tissue formed in 12 weeks and the scaffold itself degraded at the same time. Thus, 10BHP-gel scaffold may become a potential bone graft for large segmental bone defect healing in the future.
大块骨缺损的修复仍然是临床面临的一大挑战，因此对适用于该目的的合成骨移植物仍有很高的需求。本文开发了由生物活性空心纳米颗粒和交联明胶组成的亲水性复合支架（生物活性空心颗粒（BHP）-凝胶支架）。生物活性纳米颗粒具有多孔结构和高比表面积；因此，它们与明胶强烈相互作用，克服了亲水聚合物支架通常会面临的溶胀问题。通过这种组合，这些 BHP-凝胶支架表现出与松质骨相似的多孔结构和机械性能。它们在体外也表现出优异的生物活性和细胞生长促进性能。其中最好的是 10BHP-凝胶支架，在大鼠股骨模型中进行了体内评估，结果发现 5mm 节段性骨缺损在 12 周内几乎愈合，新骨组织形成，同时支架本身降解。因此，10BHP-凝胶支架将来可能成为治疗大块骨缺损的潜在骨移植物。
