Gholipour Hojjat, Meimandi-Parizi Abdolhamid, Oryan Ahmad, Bigham Sadegh Amin
Division of Surgery, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Cell Tissue Bank. 2018 Sep;19(3):341-356. doi: 10.1007/s10561-017-9679-5. Epub 2017 Dec 20.
Fibrin-platelet glue (FPG) is a blood derivative, in which platelets and fibrinogen are concentrated in a small plasma volume, by differential centrifugation and precipitation. It can form a three-dimensional and biocompatible fibrin scaffold with a myriad of growth factors and proteins that are released progressively to the local environment and contribute to the accelerated postoperative bone healing. Gelatin (Gel) is a derivative of collagen and can promote cell adhesion and proliferation due to its unique sequence of amino acids, so it is suitable for bone tissue applications. This study examined the effects of Gel, FPG and their combinations as bone scaffold on the healing of surgically created critical-size defects in rat radius. Fifty critical size defects of 5 mm long were bilaterally created in the radial diaphysis of 25 rats. The animals were randomly divided into five equal groups as empty defect, autograft, Gel, FPG and Gel-FPG groups (n = 10 in each group). Radiographs of each forelimb were taken postoperatively on the 1st day and then at the 28th and 56th days post injury to evaluate bone formation, union and remodeling of the defect. After 56 days, the rats were euthanized and their harvested healing bone samples were evaluated by histopathology, scanning electron microscopy (SEM) and biomechanical testing. The results of present study showed that the Gel alone did not significantly affect bone healing and regeneration; however, the Gel treated defects promoted healing more than those that were left untreated (negative control). Furthermore, the FPG-enhanced grafts provided a good scaffold containing numerous growth factors for proliferation of osteoinduction and was effective in improving the structural and functional properties of the newly formed bone more than that of the untreated and also the Gel treated groups. Incorporation of Gel into the FPG scaffold improved healing potential of the FPG scaffold; however, it was still inferior to the autograft (positive control). Although the Gel-FPG scaffolds had best effectiveness during bone regeneration, it still needs to be further enhanced by incorporation of the ceramic and osteoinductive biomaterials.
纤维蛋白 - 血小板胶（FPG）是一种血液衍生物，通过差速离心和沉淀法将血小板和纤维蛋白原浓缩在少量血浆中。它可以形成三维且生物相容的纤维蛋白支架，其中含有大量生长因子和蛋白质，这些物质会逐渐释放到局部环境中，有助于加速术后骨愈合。明胶（Gel）是胶原蛋白的衍生物，由于其独特的氨基酸序列，能够促进细胞黏附和增殖，因此适用于骨组织应用。本研究考察了明胶、纤维蛋白 - 血小板胶及其组合作为骨支架对大鼠桡骨手术造成的临界尺寸骨缺损愈合的影响。在25只大鼠的桡骨干中段双侧制造5毫米长的50个临界尺寸骨缺损。将动物随机分为五组，每组10只，分别为空缺损组、自体移植组、明胶组、纤维蛋白 - 血小板胶组和明胶 - 纤维蛋白 - 血小板胶组。术后第1天以及伤后第28天和第56天拍摄每个前肢的X线片，以评估骨缺损的形成、愈合和重塑情况。56天后，对大鼠实施安乐死，通过组织病理学、扫描电子显微镜（SEM）和生物力学测试对采集的愈合骨样本进行评估。本研究结果表明，单独使用明胶对骨愈合和再生没有显著影响；然而，与未处理的缺损（阴性对照）相比，经明胶处理的缺损促进了愈合。此外，纤维蛋白 - 血小板胶增强移植物提供了一个含有众多生长因子的良好支架，有利于骨诱导增殖，并且在改善新形成骨的结构和功能特性方面比未处理组和明胶处理组更有效。将明胶加入纤维蛋白 - 血小板胶支架中提高了纤维蛋白 - 血小板胶支架的愈合潜力；然而，它仍然不如自体移植（阳性对照）。尽管明胶 - 纤维蛋白 - 血小板胶支架在骨再生过程中效果最佳，但仍需要通过加入陶瓷和骨诱导生物材料来进一步增强其效果。
