Rasinhas Arthur da Costa, Silva Marcos Alexandre Nunes da, Caldas Gabriela Cardoso, Jácome Fernanda Cunha, Leonardo Raphael, Santos Flávia Barreto Dos, Nunes Priscila Conrado Guerra, Barth Ortrud Monika, Barreto-Vieira Debora Ferreira
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Imunologia Viral, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2018 Feb 5;113(4):e170208. doi: 10.1590/0074-02760170208.
The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4.
缺乏用于登革热发病机制研究的实验动物模型是疫苗和药物研发的一个限制因素。在先前的研究中，我们团队证明了BALB/c小鼠对登革病毒（DENV）1型和2型感染易感，并且该病毒在多个器官中成功分离。在本研究中，通过静脉注射使BALB/c小鼠感染DENV-4，并收集其唾液样本。从唾液样本中提取的病毒RNA进行了qRT-PCR检测，检测限为0.002 PFU/mL。在两只小鼠的唾液中检测到了DENV-4病毒RNA，病毒滴度为109 RNA/mL。在登革热诊断中，通过唾液采样检测DENV RNA并非常见做法，因为在人类患者中的检测率较低。然而，本研究观察到的结果似乎表明，与人类一样，小鼠唾液中DENV RNA的检测率也很低，这在两种情况下的感染中具有相关性。本研究报告了首次在经实验感染非神经适应性DENV-4的具有免疫能力的BALB/c小鼠唾液中检测到DENV。
