Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto14040-903, São Paulo, Brasil.
Virol J. 2010 Jan 27;7:22. doi: 10.1186/1743-422X-7-22.
Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.
早期诊断登革病毒（DENV）感染对于患者管理和登革热疫情控制非常重要。本研究的目的是分析实时 RT-PCR 法检测尿液和唾液样本在早期诊断 DENV 感染中的作用。我们选取了在圣保罗大学里贝朗普雷图医学院附属医院就诊的 2 例发热患者进行研究。采集这 2 例患者的血清、尿液和唾液样本，应用实时 RT-PCR 法检测 DENV 并进行定量分析。我们在这 2 例患者的血清、尿液和唾液样本中均检测到了登革病毒 RNA。患者 1 感染的是 DENV-2，患者 2 感染的是 DENV-3。本研究数据提示，在难以获得血液样本时，如新生儿和伴有出血综合征的患者，尿液和唾液可作为早期诊断登革病毒感染的替代样本。
