School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Food Chem. 2018 Jun 1;250:30-36. doi: 10.1016/j.foodchem.2018.01.037. Epub 2018 Jan 4.
A screening method was explored for simultaneous analysis of 43 cephalosporins residues and their metabolites from four generations in shrimp utilizing a novel fully untargeted data acquisition method. Four generations of cephalosporins residues and their metabolites are extracted with online extraction simultaneously. Ultrahigh-performance liquid chromatography coupled to quadrupole-orbitrap high resolution mass spectrometry was used for to determine cephalosporins and their metabolites in ten shrimp species. Precision in terms of relative standard deviation (RSD) was under 6.3% for all compounds, and the extraction recoveries ranged from 85% to 108%. The detection capabilities ranged from 0.011 to 0.223 mg kg. The fully untargeted vDIA acquisition way improves both selectivity and sensitivity for the compounds at trace levels, which is beneficial for screening performance.
本研究探索了一种新的、完全非靶向的数据采集方法，用于同时分析虾中四代 43 种头孢菌素及其代谢物的残留。利用在线提取，可同时提取四代头孢菌素及其代谢物残留。采用超高效液相色谱-四极杆轨道阱高分辨质谱联用技术，可测定十种虾类中的头孢菌素及其代谢物。所有化合物的相对标准偏差（RSD）均小于 6.3%，提取回收率在 85%至 108%之间。检测限范围为 0.011 至 0.223 mg/kg。完全非靶向 vDIA 采集方式提高了痕量化合物的选择性和灵敏度，有利于筛选性能。
