Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, 08193 Bellaterra, Spain.
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, 08193 Bellaterra, Spain.
Biosens Bioelectron. 2018 May 15;105:95-102. doi: 10.1016/j.bios.2018.01.024. Epub 2018 Jan 12.
The development of highly sensitive and selective enantiomeric platforms towards the rapid screening of active pharmaceutical ingredients (APIs) is nowadays a crucial challenge in several fields related to pharmacology, biomedicine, biotechnology and (bio)sensors. Herein, it is presented a novel, facile and generic methodology focused on exploiting the synergistically and electrocatalytic properties of chiral magnetic-nanobiofluids (mNBFs) with electrochemical enantiobiosensing at a magneto nanocomposite graphene paste electrode (mNC-GPE). The feasibility of this approach has been validated by chirally recognizing tryptophan (TRP) enantiomers as a proof-of-concept. For this aim, a specific chiral mNBF based on an aqueous dispersion of cobalt ferrite loaded with gold nanoparticles carrying a thiolated β-cyclodextrin (β-CD-SH/Au/CoFeO-NPs) has been synthesized and used towards the supramolecular discrimination of TRP enantiomers at an advanced graphene-paste transducer via cyclic voltammetry. This strategy, which is the first demonstration of applicability of chiral mNBFs for electrochemical enantiorecognition, opens up new approaches into enantio(bio)sensing.
现今，在药理学、生物医学、生物技术和（生物）传感器等多个领域中，开发针对活性药物成分（APIs）的高灵敏度和选择性对映体平台是一项至关重要的挑战。本文提出了一种新颖、简便且通用的方法，该方法侧重于利用手性磁性纳米生物流体（mNBFs）与在磁纳米复合石墨烯糊电极（mNC-GPE）上的电化学对映体生物传感的协同和电催化特性。通过手性识别色氨酸（TRP）对映体作为概念验证，验证了这种方法的可行性。为此，合成了一种基于钴铁氧体负载载金纳米粒子的手性 mNBF，该纳米粒子带有巯基-β-环糊精（β-CD-SH/Au/CoFeO-NPs），并将其用于通过循环伏安法在先进的石墨烯糊传感器上对 TRP 对映体进行超分子区分。这种策略是手性 mNBF 用于电化学对映体识别的首次应用实例，为对映体（生物）传感开辟了新途径。
