Watanabe T
Hokkaido Igaku Zasshi. 1986 Mar;61(2):195-207.
Human thymocytes were separated into four fractions using a technique with discontinuous bovine serum albumin (BSA) gradient centrifugation. They are fraction I (BSA 10-14%), II (14-16%), III (16-18%), IV (18-20%) and V (20-24%). Each fractions were analized their phenotype, IL2 productivity, IL2 responsibility, responder capacity in lectin induced blast genesis, helper activity and suppressor activity on immunoglobulin synthesis of peripheral blood lymphocytes stimulated with PWM in vitro. In addition, the effect of phorbol myristate acetate (PMA) on thymocyte fractions was analized. Human thymocytes were separated into three subpopulations according to their stage of differentiation and maturation. They were as follows; (1) mature T lymphocytes, fraction I and II, which produced IL2, responded to IL2 and had both suppressor and helper function; (2) immature thymocytes, fraction III and a part of IV, which responded to IL2 without production of IL2 and had neither suppressor nor helper function, but suppressor function was induced by Con A with IL2 from this group of thymocytes; (3) immature thymocytes, a major part of fraction IV, which had no IL2 production and no responsibility, and had neither suppressor nor helper function. PMA enhanced PHA induced IL2 production and IL2 responsibility of human thymocytes, and also PMA induced suppressor function from human thymocytes.
使用不连续牛血清白蛋白（BSA）梯度离心技术将人胸腺细胞分离为四个组分。它们分别是组分I（BSA 10 - 14%）、II（14 - 16%）、III（16 - 18%）、IV（18 - 20%）和V（20 - 24%）。对每个组分的表型、IL2产生能力、对IL2的反应性、凝集素诱导的母细胞化反应能力、辅助活性以及对体外经PWM刺激的外周血淋巴细胞免疫球蛋白合成的抑制活性进行了分析。此外，还分析了佛波酯肉豆蔻酸酯（PMA）对胸腺细胞组分的影响。根据分化和成熟阶段，人胸腺细胞被分为三个亚群。具体如下：（1）成熟T淋巴细胞，即组分I和II，它们产生IL2，对IL2有反应，并且具有抑制和辅助功能；（2）未成熟胸腺细胞，组分III和部分IV，它们对IL2有反应但不产生IL2，既无抑制功能也无辅助功能，但该组胸腺细胞中的Con A与IL2可诱导其产生抑制功能；（3）未成熟胸腺细胞，组分IV的主要部分，它们不产生IL2且无反应性，既无抑制功能也无辅助功能。PMA增强了PHA诱导的人胸腺细胞IL2产生和对IL2的反应性，并且PMA还诱导了人胸腺细胞的抑制功能。
