Sakiyama Y, Ishizaka A, Watanabe T, Ariga T, Matsumoto S
Department of Pediatrics, Hokkaido University School of Medicine, Sapporo.
Tohoku J Exp Med. 1988 Feb;154(2):195-203. doi: 10.1620/tjem.154.195.
Human thymocytes were separated according to differential agglutination by peanut lectin (PNA). In the fractions obtained, the distribution and quantitative expression of CD antigens was determined by two-color fluorescence flow cytometry. A major population of CD4+8+ and minor populations of CD4+8- and CD4-8+ cells existed in unfractionated thymocytes. Agglutination of human thymocytes by PNA led to a highly effective enrichment of the CD4+8+ phenotype in the PNA+ thymocytes, and left three distinct phenotypes, CD4+8+, CD4+8-, and CD4-8+, in the PNA-thymocytes. After treatment of the CD4+8+ cells in both PNA+ and PNA- fractions for 20 hr with phorbol 12-myristate 13-acetate (PMA), 60%-90% of the cells expressed the CD4-8+ phenotype, whereas the CD4+8+ phenotype was decreased to 1%-3% of the population. In addition, pretreatment of both PNA+ and PNA- thymocytes with PMA induced suppressor activity in these cells, as shown by inhibition of immunoglobulin secretion by pokeweed mitogen (PWM)-stimulated peripheral blood lymphocytes (PBL).
人胸腺细胞通过花生凝集素（PNA）的差异凝集进行分离。在所获得的组分中，通过双色荧光流式细胞术测定CD抗原的分布和定量表达。未分离的胸腺细胞中存在主要的CD4 + 8 +细胞群以及次要的CD4 + 8 -和CD4 - 8 +细胞群。PNA对人胸腺细胞的凝集导致PNA +胸腺细胞中CD4 + 8 +表型的高效富集，并在PNA -胸腺细胞中留下三种不同的表型，即CD4 + 8 +、CD4 + 8 -和CD4 - 8 +。用佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA）对PNA +和PNA -组分中的CD4 + 8 +细胞处理20小时后，60% - 90%的细胞表达CD4 - 8 +表型，而CD4 + 8 +表型减少至群体的1% - 3%。此外，如通过抑制商陆有丝分裂原（PWM）刺激的外周血淋巴细胞（PBL）的免疫球蛋白分泌所示，用PMA对PNA +和PNA -胸腺细胞进行预处理会诱导这些细胞中的抑制活性。
