微波辐射下获得的可生物降解、pH 敏感的壳聚糖珠，用于高级细胞培养。
Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture.
机构信息
Department of Biotechnology and Physical Chemistry, Cracow University of Technology, Cracow, Poland.
Department of Biotechnology and Physical Chemistry, Cracow University of Technology, Cracow, Poland.
出版信息
Colloids Surf B Biointerfaces. 2018 Apr 1;164:324-331. doi: 10.1016/j.colsurfb.2018.01.061. Epub 2018 Feb 1.
A new type of promising chitosan beads with advanced properties were obtained under microwave radiation according to Green Chemistry principles. Biomaterials were prepared using chitosan as raw material and glutamic acid/1,5-pentanodiol mixture as crosslinking agents. Additionally beads were modified with Tilia platyphyllos extract to enhance their antioxidant properties. Beads were investigated over their chemical structure by FT-IR analysis. Also their morphology has been investigated by SEM method. Additionally swelling capacity of the obtained hydrogels was determined. Lack of cytotoxicity has been confirmed by MTT assay. Proliferation studies were carried out on L929 mouse fibroblasts. Advanced properties of the obtained beads were investigated by studying pH sensitivity and antioxidant properties by DPPH method. Also susceptibility to degradation and biodegradation by Sturm Test method was evaluated. Results shows that proposed chitosan beads and their eco-friendly synthesis method can be applied in cell therapy and tissue engineering.
根据绿色化学原则，在微波辐射下获得了一种具有先进性能的新型有前途的壳聚糖珠。生物材料是用壳聚糖作为原料，谷氨酸/1,5-戊二醇混合物作为交联剂制备的。此外，还使用椴树提取物对珠子进行改性以提高其抗氧化性能。通过傅里叶变换红外（FT-IR）分析研究了珠子的化学结构。还通过扫描电子显微镜（SEM）方法研究了它们的形态。此外，还测定了所得水凝胶的溶胀能力。通过 MTT 测定法证实了无细胞毒性。在 L929 小鼠成纤维细胞上进行了增殖研究。通过 DPPH 法研究了所得到的珠子的 pH 敏感性和抗氧化性能，评估了通过 Sturm 测试法对降解和生物降解的敏感性，来研究其先进的性能。结果表明，所提出的壳聚糖珠及其环保合成方法可应用于细胞治疗和组织工程。
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