新型壳聚糖基支架在经组织工程治疗盆腔器官脱垂（POP）中的潜力。
The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering.
机构信息
Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, Poland.
Gynecology and Oncology Department Jagiellonian University Collegium Medicum, Kopernika 23, 31-501 Kraków, Poland.
出版信息
Molecules. 2020 Sep 18;25(18):4280. doi: 10.3390/molecules25184280.
The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including l-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT-IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment.
越来越多的女性生殖系统疾病需要新的治疗方法。组织工程为患者带来了希望，它可以实现受损组织的重建。为此，上皮细胞在三维支架上培养。壳聚糖是最有前途的材料之一，它具有生物相容性和可生物降解性。本研究旨在验证基于壳聚糖的生物材料在盆腔器官脱垂再生方面的潜力。支架是在微波辅助条件下通过交联反应获得的，使用二羧酸和氨基酸作为交联剂，包括 l-谷氨酸、己二酸、丙二酸和乙酰丙酸。对产物进行了理化和生物学性质的表征。FT-IR 分析证实了酰胺键的形成。支架具有高度多孔的结构，这通过 SEM 分析得到了证实。它们的孔隙率超过 90%。生物材料具有优异的溶胀能力和非常好的抗氧化性能。细胞毒性研究在阴道上皮 VK2/E6E7 和人结肠癌细胞 HCT116 上进行。结果表明，经过某些修饰后，所提出的支架可用于治疗盆腔器官脱垂 (POP)。
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