藏红花素对宫颈癌（HeLa 细胞）的抗癌活性：藏红花素在雄性白化大鼠中的生物评估和毒性评价。
Anticancer activity of crocin against cervical carcinoma (HeLa cells): Bioassessment and toxicity evaluation of crocin in male albino rats.
机构信息
Clinic laboratory of Zhuzhou Central Hospital, Zhuzhou city, Hunan Province, China, 412000.
Clinic laboratory of Zhuzhou Central Hospital, Zhuzhou city, Hunan Province, China, 412000.
出版信息
J Photochem Photobiol B. 2018 Mar;180:118-124. doi: 10.1016/j.jphotobiol.2018.01.013. Epub 2018 Jan 17.
The present study was aimed to investigate anticancer activity of crocin against cervical carcinoma and bio-assessment and toxicological evaluation in male albino rats. Effect of crocin on cell viability (anticancer activity) was determined against cervical carcinoma cells. Chronic effect of crocin on body weight changes, serum enzymes, serum biochemical markers, lipid peroxidation, hematological markers and DNA damage in male albino rats were determined. Cell survival rate was reduced 98.4, 95.7, 87.2, 81.1 and 73.1% at 25, 50, 75, 100 and 125 mg/l of crocin respectively. Cell viability was reduced 97.1, 96.4, 85.5, 78.4 and 70.2% at 25, 50, 75, 100 and 125 mg/l of crocin respectively. Crocin reduced body weight significantly at 30 and 60th day. Alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine, bilirubin, albumin and total protein were decreased, while glucose, cholesterol, TG, and GSH were increased. Hemoglobin (Hb), white blood cells (WBC), lymphocytes, neutrophil and packed cell volume (PCV) were altered following crocin treatment. Necrosis, fibrosis, mononuclear infiltration, angiogenesis and DNA fragmentation were also noted. Taking all these data together, it is suggested that the crocin could be a potential antitumor agent against cervical carcinoma. However, the altered histological, biochemical and hematological markers may lead to an adverse effect on the cellular metabolism and physiological activity.
本研究旨在探讨藏红花素对宫颈癌的抗癌活性，并对雄性白化大鼠进行生物评估和毒理学评价。采用藏红花素作用于宫颈癌细胞，检测其对细胞活力（抗癌活性）的影响。测定藏红花素对雄性白化大鼠体重变化、血清酶、血清生化标志物、脂质过氧化、血液学标志物和 DNA 损伤的慢性影响。藏红花素在 25、50、75、100 和 125mg/l 时，细胞存活率分别降低了 98.4%、95.7%、87.2%、81.1%和 73.1%。藏红花素在 25、50、75、100 和 125mg/l 时，细胞活力分别降低了 97.1%、96.4%、85.5%、78.4%和 70.2%。藏红花素在第 30 天和第 60 天显著降低了体重。丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）、碱性磷酸酶（ALP）、血尿素氮（BUN）、肌酐、胆红素、白蛋白和总蛋白降低，而葡萄糖、胆固醇、TG 和 GSH 增加。血红蛋白（Hb）、白细胞（WBC）、淋巴细胞、中性粒细胞和红细胞压积（PCV）在藏红花素处理后发生改变。还观察到坏死、纤维化、单核细胞浸润、血管生成和 DNA 片段化。综合所有这些数据，表明藏红花素可能是一种有潜力的宫颈癌抗肿瘤药物。然而，改变的组织学、生化和血液学标志物可能导致对细胞代谢和生理活性的不利影响。
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