Hatziagapiou Kyriaki, Nikola Olti, Marka Sofia, Koniari Eleni, Kakouri Eleni, Zografaki Maria-Eleftheria, Mavrikou Sophie S, Kanakis Charalabos, Flemetakis Emmanouil, Chrousos George P, Kintzios Spyridon, Lambrou George I, Kanaka-Gantenbein Christina, Tarantilis Petros A
Choremeio Research Laboratory, First Department of Pediatrics, National and Kapodistrian University of Athens, Thivon & Levadeias 8, 11527 Athens, Greece.
Physiotherapy Department, Faculty of Health and Care Sciences, State University of West Attica, Agiou Spiridonos 28, Egaleo, 12243 Athens, Greece.
Antioxidants (Basel). 2022 May 28;11(6):1074. doi: 10.3390/antiox11061074.
L. has various pharmacological properties, known for over 3600 years. These properties are attributed mainly to biologically active substances, which belong to the terpenoid group and include crocins, picrocrocin and safranal. The aim of the current work was to examine the effects of crocins (CRCs) and their methyl ester derivate dimethylcrocetin (DMCRT) on glioblastoma and rhabdomyosarcoma cell lines, in terms of cytotoxicity and gene expression, implicated in proapoptotic and cell survival pathways. Cell cytotoxicity was assessed with Alamar Blue fluorescence assay after treatment with saffron carotenoids for 24, 48 and 72 h and concentrations ranging from 22.85 to 0.18 mg/mL for CRCs and 11.43 to 0.09 mg/mL for DMCRT. In addition, , , , , , and gene expression was studied by qRT-PCR analysis. Both compounds demonstrated cytotoxic effects against glioblastoma and rhabdomyosarcoma cell lines, in a dose- and time-dependent manner. They induced apoptosis, via upregulation, and , , downregulation. The current research denotes the possible anticancer properties of saffron carotenoids, which are considered safe phytochemicals, already tested in clinical trials for their health promoting properties.
藏红花已有3600多年的历史，具有多种药理特性。这些特性主要归因于生物活性物质，它们属于萜类化合物，包括藏红花素、西红花苦苷和藏红花醛。本研究的目的是从细胞毒性和基因表达方面，研究藏红花素（CRCs）及其甲酯衍生物二甲基藏红花酸（DMCRT）对胶质母细胞瘤和横纹肌肉瘤细胞系的影响，这些影响与促凋亡和细胞存活途径有关。在用藏红花类胡萝卜素处理24、48和72小时后，使用Alamar Blue荧光测定法评估细胞毒性，CRCs的浓度范围为22.85至0.18mg/mL，DMCRT的浓度范围为11.43至0.09mg/mL。此外，通过qRT-PCR分析研究了 、 、 、 、 和 基因的表达。两种化合物均对胶质母细胞瘤和横纹肌肉瘤细胞系表现出细胞毒性作用，且呈剂量和时间依赖性。它们通过上调 以及下调 、 、 诱导细胞凋亡。目前的研究表明藏红花类胡萝卜素可能具有抗癌特性，它们被认为是安全的植物化学物质，已经在临床试验中因其促进健康的特性而进行了测试。
