Vohra R, Majumdar S, Malik V, Singh R, Nagpaul J P
Indian J Physiol Pharmacol. 1985 Jul-Sep;29(3):146-52.
The effect of a single dose of 5 mg/kg body weight of aflatoxin B1 on rat liver mitochondrial enzymes, succinate dehydrogenase (SDH) and Mg++ adenosine triphosphatase (Mg++-ATPase) and on certain lipids were studies at various intervals of time from 3 to 24 hours. A significant decrease in the specific activity of SDH was observed after 6, 12, 18 and 24 hr treatment. The Mg++-ATPase activity remained unaffected up to 12 hr but appreciably decreased after, 18 and 24 hr of the treatment. The level of phospholipids and cholesterol were not altered after 3, 6 and 12 hr treatment, thereafter (18 and 24 hr) an increase was observed in both the lipids following the aflatoxin treatment. Medroxyprogesterone acetate (MPA) did not cause any alteration in the specific activities of these enzymes as well as levels of cholesterol and phospholipids. The treatment with MPA caused significant increase in contents of cytochromes P-450, b5 and activities of Arylhydrocarbon hydroxylase (AHH), UDP-glucuronyl transferase (UDP-GT) and NADPH-cytochrome C-reductase of hepatic microsomes. It was observed that pretreatment with medroxyprogesterone acetate (MPA) could significantly minimuze the depression caused in mitochondrial SDH and Mg++-ATPase activities by aflatoxin B1.
研究了单剂量5mg/kg体重的黄曲霉毒素B1在3至24小时的不同时间间隔对大鼠肝脏线粒体酶、琥珀酸脱氢酶（SDH）和Mg++-三磷酸腺苷酶（Mg++-ATPase）以及某些脂质的影响。在处理6、12、18和24小时后，观察到SDH的比活性显著降低。Mg++-ATPase活性在12小时内未受影响，但在处理18和24小时后明显降低。在处理3、6和12小时后，磷脂和胆固醇水平未改变，此后（18和24小时），黄曲霉毒素处理后两种脂质均出现增加。醋酸甲羟孕酮（MPA）对这些酶的比活性以及胆固醇和磷脂水平未引起任何改变。MPA处理导致细胞色素P-450、b5含量以及肝微粒体芳烃羟化酶（AHH）、UDP-葡糖醛酸基转移酶（UDP-GT）和NADPH-细胞色素C还原酶活性显著增加。观察到醋酸甲羟孕酮（MPA）预处理可显著减轻黄曲霉毒素B1对线粒体SDH和Mg++-ATPase活性造成的抑制。
