Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin 9, Ireland.
Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin 9, Ireland; School of Biotechnology, Dublin City University, Dublin 9, Ireland.
Biosens Bioelectron. 2018 May 30;106:78-85. doi: 10.1016/j.bios.2018.01.056. Epub 2018 Jan 31.
Assesment of human epidermal growth factor receptor 2 status is a key factor prompting definitive treatment decisions that help in reducing mortality rates associated with breast cancer. In this article, highly sensitive and low-cost impedimetric immunosensor using single-chain fragment variable antibody fragments was developed for quantitative detection of human epidermal growth factor receptor 2 from serum employing gold nanoparticle-modified disposable screen-printed carbon electrodes. The gold nanoparticles facilitate fast electron transfer and offer a biocompatible surface for immobilization of small antibody fragments in an oriented manner, resulting in improved antigen binding efficiency. The single-chain fragment variable antibody fragment-modified screen printed immunosensor exhibits wide dynamic range of 0.01-100 ng mL and detection limit of 0.01 ng mL. The advantages offered by this platform in terms of high sensitivity, broad dynamic range and low-cost demonstrates great potential for improved monitoring of human epidermal growth factor receptor 2 levels for the management of breast and other cancers.
人表皮生长因子受体 2 状态评估是促使明确治疗决策的关键因素，有助于降低乳腺癌相关死亡率。本文开发了一种基于单链抗体片段的高灵敏度、低成本的阻抗免疫传感器，用于采用金纳米粒子修饰的一次性丝网印刷碳电极从血清中定量检测人表皮生长因子受体 2。金纳米粒子促进了快速电子转移，并为小分子抗体片段的定向固定提供了一个生物相容的表面，从而提高了抗原结合效率。单链抗体片段修饰的丝网印刷免疫传感器具有 0.01-100ng/mL 的宽动态范围和 0.01ng/mL 的检测限。该平台在高灵敏度、宽动态范围和低成本方面的优势，为改善人表皮生长因子受体 2 水平监测以管理乳腺癌和其他癌症提供了巨大潜力。
