School of chemistry and chemical engineering, Jiangsu University, Zhenjiang 212013, PR China.
School of chemistry and chemical engineering, Jiangsu University, Zhenjiang 212013, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Apr 15;195:210-214. doi: 10.1016/j.saa.2018.01.076. Epub 2018 Feb 1.
In order to develop NIR BODIPY for mitochondria targeting imaging agents and metal sensors, a side chain modified BODIPY (BPN) was synthesized and spectroscopically characterized. BPN has NIR emission at 765nm when excited at 704nm. The emission at 765nm responded differently to Cu and Mn ions, respectively. The BPN coordinated with Cu forming [BPNCu] complex with quenched emission, while Mn induced aggregation of BPN with specific fluorescence enhancement. Moreover, BPN can be applied to monitor Cu in live cells and image mitochondria. Further, BPN was used as sensor for the detection of Cu ions in serum with linear detection range of 0.45μM-36.30μM. Results indicate that BPN is a good sensor for the detection of Cu in serum and image mitochondria. This study gives strategies for future design of NIR sensors for the analysis of metal ions in blood.
为了开发用于线粒体靶向成像剂和金属传感器的近红外 BODIPY，合成并光谱表征了侧链修饰的 BODIPY（BPN）。当在 704nm 处激发时，BPN 在 765nm 处具有近红外发射。在 765nm 处的发射分别对 Cu 和 Mn 离子有不同的响应。BPN 与 Cu 配位形成具有猝灭发射的[BPNCu]配合物，而 Mn 诱导 BPN 聚集并具有特定的荧光增强。此外，BPN 可用于监测活细胞中的 Cu 并对线粒体进行成像。此外，BPN 可用作血清中 Cu 离子检测的传感器，其线性检测范围为 0.45μM-36.30μM。结果表明，BPN 是一种用于检测血清中 Cu 并对线粒体进行成像的良好传感器。本研究为未来设计用于分析血液中金属离子的近红外传感器提供了策略。
