苯并(a)芘在体外对体液免疫和细胞介导免疫反应的抑制作用。

Suppression of humoral and cell-mediated immune responses in vitro by benzo(a)pyrene.

作者信息

Urso P, Gengozian N, Rossi R M, Johnson R A

出版信息

J Immunopharmacol. 1986;8(2):223-41. doi: 10.3109/08923978609028616.

Abstract

The effect of benzo(a)pyrene (BaP) at different molar (M) concentrations on the in vitro anti-sheep red blood cell (SRBC) plaque (antibody) forming cell (PFC) response and the one-way mixed lymphocyte response (MLR) was tested. Inhibition of the PFC response and the MLR occurred when spleen cells were exposed to a wide range of BaP concentrations from 10(-4) M to 10(-8) M. Maximum depression of the responses occurred at 10(-5) M for PFC production (47% of controls) and for the MLR (19% of controls) as measured by a stimulation index. No significant loss in cell viability was observed at this or lower molar concentrations of BaP. The non-carcinogenic analog of BaP, benzo(e)pyrene, did not suppress PFC responses at comparable concentrations. This in vitro system will facilitate manipulations of T and B lymphocytes and macrophages (adherent cells) in a controlled culture environment for precisely characterizing the sensitivity of these cells and their subpopulations on exposure to BaP.

摘要

测试了不同摩尔（M）浓度的苯并（a）芘（BaP）对体外抗绵羊红细胞（SRBC）斑块（抗体）形成细胞（PFC）反应和单向混合淋巴细胞反应（MLR）的影响。当脾细胞暴露于10⁻⁴ M至10⁻⁸ M的广泛BaP浓度范围时，PFC反应和MLR受到抑制。通过刺激指数测量，PFC产生和MLR的最大反应抑制分别出现在10⁻⁵ M时（分别为对照的47%和19%）。在该摩尔浓度或更低浓度的BaP下，未观察到细胞活力有显著损失。BaP的非致癌类似物苯并（e）芘在可比浓度下未抑制PFC反应。这种体外系统将有助于在可控培养环境中对T和B淋巴细胞以及巨噬细胞（贴壁细胞）进行操作，以精确表征这些细胞及其亚群在暴露于BaP时的敏感性。