Yang Ming-Hui, Chen Wan-Jou, Fu Yaw-Syan, Huang Bin, Tsai Wan-Chi, Arthur Chen Yi-Ming, Lin Po-Chiao, Yuan Cheng-Hui, Tyan Yu-Chang
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Oncotarget. 2017 Dec 7;9(1):442-452. doi: 10.18632/oncotarget.23064. eCollection 2018 Jan 2.
Glycine -methyltransferase is a tumor suppressor gene for hepatocellular carcinoma, which can activate DNA methylation by inducing the -adenosylmethionine to -adenosylhomocystine. Previous studies have indicated that the expression of Glycine -methyltransferase is inhibited in hepatocellular carcinoma. To confirm and identify missing proteins, the pathologic analysis of the tumor-bearing mice will provide critical histologic information. Such a mouse model is applied as a screening tool for hepatocellular carcinoma as well as a strategy for missing protein discovery. In this study we designed an analysis platform using the human proteome atlas to compare the possible missing proteins to human whole chromosomes. This will integrate the information from animal studies to establish an optimal technique in the missing protein biomarker discovery.
甘氨酸甲基转移酶是一种肝细胞癌的肿瘤抑制基因，它可通过诱导S-腺苷甲硫氨酸转化为S-腺苷同型半胱氨酸来激活DNA甲基化。先前的研究表明，甘氨酸甲基转移酶的表达在肝细胞癌中受到抑制。为了确认和鉴定缺失的蛋白质，对荷瘤小鼠进行病理分析将提供关键的组织学信息。这样的小鼠模型既作为肝细胞癌的筛选工具，也是发现缺失蛋白质的一种策略。在本研究中，我们设计了一个利用人类蛋白质组图谱的分析平台，将可能缺失的蛋白质与人类全染色体进行比较。这将整合来自动物研究的信息，以建立一种在发现缺失蛋白质生物标志物方面的最佳技术。
