Blair W F, Chang L, Pedersen D R, Gabel R H, Bell L D
Microsurgery. 1986;7(2):84-6. doi: 10.1002/micr.1920070207.
This investigation initiates the quantitative, hemodynamic assessment of interpositional grafts in small rat arteries. The left femoral arteries in 16 Sprague-Dawley rats were transected at two levels, and were then repaired using interrupted suturing technique. This effectively provided an ideally sized and histologically matched interposition graft. The 20-MHz PUDVM method was used to measure blood velocities, and derive values for vessel lumen area, temporal mean of the spatial mean velocity (Vsm), and volumetric flows. Measurements were completed distal to the interposed graft. Variables were quantitated in the preoperative and at the 5-, 15-, and 30-minute postoperative intervals. Statistical analysis of data indicated that the interpositional grafting procedure resulted in increased vessel lumen area and decreased Vsm, but, importantly, volumetric flow (about 8.20 ml/min) remained unchanged. This study demonstrated that, although hemodynamic characteristics are altered, volumetric flow can be restored after an experimental, interpositional grafting procedure in small arteries.
本研究开始对大鼠小动脉间置移植血管进行定量血流动力学评估。将16只Sprague-Dawley大鼠的左股动脉在两个水平切断，然后用间断缝合技术进行修复。这有效地提供了理想尺寸且组织学匹配的间置移植物。采用20MHz脉冲多普勒超声心动图（PUDVM）方法测量血流速度，并得出血管腔面积、空间平均速度的时间平均值（Vsm）和容积流量的值。测量在间置移植物远端完成。在术前以及术后5分钟、15分钟和30分钟对变量进行定量。数据的统计分析表明，间置移植手术导致血管腔面积增加，Vsm降低，但重要的是，容积流量（约8.20 ml/min）保持不变。本研究表明，尽管血流动力学特征发生改变，但在小动脉进行实验性间置移植手术后，容积流量可以恢复。
