Eraghi Vida, Derakhshandeh Abdollah, Hosseini Arsalan, Motamedi-Boroojeni Azar
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Mol Biol Res Commun. 2017 Dec;6(4):161-168. doi: 10.22099/mbrc.2017.26522.1286.
subsp. (MAP) is the etiologic agent of Johne's disease in ruminants and there has been a shift in the public health approach to MAP and human diseases like Crohn's disease. The prevention of infection by MAP in ruminants is thought to deter the high impact of economic losses in the level of dairy industry and possible spreading of this pathogen in dairy products. The present study was done to investigate the construction and expression of the soluble form of a novel fusion protein, consisting of Heparin-binding hemagglutinin (HBHA) and high antigenic region of Fibronectin Attachment Protein-P (FAP-P), in order to introduce as a Th1 inducer subunit vaccine against MAP. HBHA is a mycobacterial adhesin and it has been demonstrated that a HBHA-specific IFN-γ response, in latent infection, depends on the methylation of the antigen. Further, induces Th1 polarization. Because methylation of HBHA was not performed in , was chosen as the host. The desired fusion protein had a similar 3D structure to that of HBHA with its native form and post-translational methylation in C-terminal. Hence, the uptake of the purified fusion protein will be done by M cells because of HBHA, and cell-mediated immunity will be induced because of both antigens. Eventually, successful construction and expression of the newly-designed chimeric protein under the mentioned conditions is reported in this article.
副结核分枝杆菌亚种（MAP）是反刍动物约翰氏病的病原体，并且在公共卫生领域对MAP以及像克罗恩病这类人类疾病的应对方式上已经出现了转变。反刍动物感染MAP的预防被认为可以抑制乳制品行业经济损失的巨大影响以及这种病原体在乳制品中的可能传播。本研究旨在调查一种新型融合蛋白可溶性形式的构建和表达，该融合蛋白由肝素结合血凝素（HBHA）和纤连蛋白附着蛋白-P（FAP-P）的高抗原区组成，以便作为一种针对MAP的Th1诱导亚单位疫苗引入。HBHA是一种分枝杆菌粘附素，并且已经证明在潜伏感染中，HBHA特异性的IFN-γ反应取决于抗原的甲基化。此外，其诱导Th1极化。由于在[未提及具体内容]中未进行HBHA的甲基化，[未提及具体内容]被选作宿主。所需的融合蛋白在其天然形式下具有与HBHA相似的三维结构，并且在C端具有翻译后甲基化。因此，由于HBHA，纯化的融合蛋白将被M细胞摄取，并且由于两种抗原将诱导细胞介导的免疫。最终，本文报道了在上述条件下新设计的嵌合蛋白的成功构建和表达。
