Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat, Egypt.
Inflammation. 2018 Jun;41(3):760-772. doi: 10.1007/s10753-018-0730-x.
Thermal injury may lead to multiple organ dysfunction through release of proinflammatory mediators and reactive oxygen radicals. This study investigated the effects of thermal injury on remote organs of rats and the possible protective effect of lutein. Thermal trauma was induced in the back of rats by exposing them to 90 °C bath for 10 s. Rats were sacrificed 48 h after burn, and blood samples were collected to monitor liver and kidney functions. Tissue samples from liver, kidneys, and lungs were taken for studying oxidative stress parameters, gene expressions of TNF-α and Casp-3, besides histopathological examination. Skin scald injury caused significant elevations of liver and kidney function biomarkers in the serum. In tissue samples, increments of MDA, GPx, and 8-OHdG were recorded while GSH level and the activities of CAT and SOD were suppressed. The expressions of TNF-α and caspase-3 mRNA were increased, and histopathological results revealed remote organ injury. Oral administration of lutein (250 mg/kg) resulted in amelioration of the biochemical and molecular changes induced by burn as well as the histopathological alterations. According to the findings of the present study, lutein possesses anti-oxidant, anti-inflammatory, and anti-apoptotic effects that protect against burn-induced damage in remote organs.
热损伤可通过释放促炎介质和活性氧自由基导致多器官功能障碍。本研究探讨了热损伤对大鼠远隔器官的影响，以及叶黄素可能的保护作用。通过将大鼠暴露于 90°C 的浴中 10 秒来诱导热创伤。烧伤后 48 小时处死大鼠，采集血样以监测肝肾功能。采集肝、肾和肺组织样本，以研究氧化应激参数、TNF-α 和 Caspase-3 的基因表达，以及组织病理学检查。皮肤烫伤损伤导致血清中肝肾功能生物标志物显著升高。在组织样本中，记录到 MDA、GPx 和 8-OHdG 的增加，而 GSH 水平以及 CAT 和 SOD 的活性受到抑制。TNF-α 和 caspase-3 mRNA 的表达增加，组织病理学结果显示远隔器官损伤。叶黄素（250mg/kg）的口服给药可改善烧伤引起的生化和分子变化以及组织病理学改变。根据本研究的结果，叶黄素具有抗氧化、抗炎和抗细胞凋亡作用，可防止远隔器官的烧伤损伤。
