Hladovec J
Physiol Bohemoslov. 1986;35(2):97-103.
The endothelo-protective activity of a series of low-molecular oxygen-derived free radical scavengers (OFRS) was tested in rats. A model of endothelaemia provoked by intravenous administration of hydrogen peroxide was used. With each OFRS the activity in the hydrogen peroxide model was compared with that in the less specific model using the provocation by citrate as a calcium chelating agent. Relatively unspecific but biologically important OFRS, ascorbic acid, tocopherol, troxerutin and glutathione were tested in the first phase of the study. A marked optimum of endothelo-protective activity was shown with all agents, the optimum against hydrogen peroxide having been observed at doses from 3 to 50 times lower than against citrate. Ascorbic acid, troxerutin and the combination of both were also tested in another model based on leg ischaemia produced by ligature of the common femoral artery. Without OFRS, a marked increase of endothelaemia was observed after 30-60 min ischaemia showing a second peak after the release of the ligature. This second peak was completely abolished by the preventive administration of OFRS in a dose which was also effective in the hydrogen peroxide model.
在大鼠中测试了一系列低分子氧衍生自由基清除剂（OFRS）的内皮保护活性。使用了通过静脉注射过氧化氢引发的内皮血症模型。对于每种OFRS，将其在过氧化氢模型中的活性与在使用柠檬酸盐作为钙螯合剂激发的不太特异的模型中的活性进行比较。在研究的第一阶段测试了相对非特异性但具有生物学重要性的OFRS，如抗坏血酸、生育酚、曲克芦丁和谷胱甘肽。所有试剂均显示出明显的内皮保护活性最佳值，对抗过氧化氢的最佳剂量比对抗柠檬酸盐的剂量低至3至50倍。抗坏血酸、曲克芦丁及其组合也在另一个基于股总动脉结扎产生的腿部缺血的模型中进行了测试。在没有OFRS的情况下，缺血30 - 60分钟后观察到内皮血症明显增加，在松开结扎后出现第二个峰值。通过预防性给予在过氧化氢模型中也有效的剂量的OFRS，完全消除了这个第二个峰值。
