Huot R I, Shain S A
Cancer Res. 1986 Aug;46(8):3775-81.
We examined androgen modulation of proliferation of clonally derived AXC/SSh rat prostate cancer cells. C-family cells were maintained on medium without addition of androgens. D-family cells were maintained on medium containing 10(-7) M 5 alpha-dihydrotestosterone and T-family cells were maintained on medium containing 10(-7) M testosterone. Proliferation of all AXC/SSh prostate cancer cell lines during propagation on media containing fetal bovine serum was not altered by changes in media testosterone concentration through the range 10(-6) to 10(-9) M. Similarly, proliferation of C- or D-family cell lines, during propagation on media containing steroid depleted, charcoal stripped fetal bovine serum, was not altered by changes in media testosterone concentration through the range 10(-6) to 10(-9) M. By contrast, proliferation of T-family cell lines during propagation on charcoal stripped fetal bovine serum was modulated by androgens; effects were androgen concentration dependent and maximum at 10(-8) to 10(-7) M. Androgens decreased T5 cell proliferation rate and diminished achievable saturation density, whereas T1 cell proliferation rate was increased by androgens. In contrast, T6 cell proliferation rate was unaffected by androgens; however, saturation density was increased. Effects were antagonized by the antiandrogen RU 23908, Anandron, establishing androgen specificity of testosterone or 5 alpha-dihydrotestosterone mediated changes in proliferation.
我们研究了雄激素对克隆衍生的AXC/SSh大鼠前列腺癌细胞增殖的调节作用。C家族细胞在不添加雄激素的培养基中培养。D家族细胞在含有10^(-7) M 5α-二氢睾酮的培养基中培养，T家族细胞在含有10^(-7) M睾酮的培养基中培养。在含有胎牛血清的培养基中传代培养时，所有AXC/SSh前列腺癌细胞系的增殖不受培养基睾酮浓度在10^(-6)至10^(-9) M范围内变化的影响。同样，在含有经类固醇去除、活性炭处理的胎牛血清的培养基中传代培养时，C或D家族细胞系的增殖也不受培养基睾酮浓度在10^(-6)至10^(-9) M范围内变化的影响。相比之下，T家族细胞系在经活性炭处理的胎牛血清中传代培养时，其增殖受到雄激素的调节；这种作用呈雄激素浓度依赖性，在10^(-8)至10^(-7) M时达到最大值。雄激素降低了T5细胞的增殖速率并降低了可达到的饱和密度，而T1细胞的增殖速率则因雄激素而增加。相比之下，T6细胞的增殖速率不受雄激素影响；然而，其饱和密度增加。这些作用被抗雄激素RU 23908（阿南朵）所拮抗，证实了睾酮或5α-二氢睾酮介导的增殖变化具有雄激素特异性。
