Shain S A, Huot R I
Department of Cellular and Molecular Biology, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.
J Steroid Biochem. 1987;27(1-3):503-12. doi: 10.1016/0022-4731(87)90347-5.
We used heterogeneous parental cultures of AXC/SSh rat prostate cancer cells to isolate clonally derived prostate cancer cell lines. Light and electron microscopic analyses established that parental and clonally isolated cells possess features characteristic of secretory epithelium. Biochemical analyses showed that these cells contained androgen receptors and acid phosphatase and 5 alpha-reductase activity; phenotypic markers characteristic of differentiated prostate epithelium. Content of these prostate epithelial cell markers was variable and cell line specific. We used selected cell lines to examine androgen modulation of AXC/SSh rat prostate cancer cell proliferation in vitro. We found that proliferation of C-family or D-family cells, those respectively maintained on medium without additions or medium containing 10(-7) M 5 alpha-dihydrotestosterone, was not affected by changes in medium testosterone concentration through the range 10(-6)-10(-9) M. In contrast, testosterone modified proliferation of T-family cells, those maintained on medium containing 10(-7) M testosterone, and effects were antagonized by the anti-androgen RU 23908. Preliminary studies established that AXC/SSh rat prostate cancer cells elaborate polypeptide components which stimulate in vitro cell proliferation. Both the ability to elaborate these components and their effects on in vitro cell proliferation appeared to be cell line specific.
我们使用AXC/SSh大鼠前列腺癌细胞的异质亲本培养物来分离克隆衍生的前列腺癌细胞系。光学和电子显微镜分析表明，亲本细胞和克隆分离的细胞具有分泌上皮的特征。生化分析表明，这些细胞含有雄激素受体、酸性磷酸酶和5α-还原酶活性；这些是分化前列腺上皮的表型标志物。这些前列腺上皮细胞标志物的含量是可变的，且具有细胞系特异性。我们使用选定的细胞系来研究雄激素对AXC/SSh大鼠前列腺癌细胞体外增殖的调节作用。我们发现，C家族或D家族细胞（分别在无添加物的培养基或含有10^(-7) M 5α-双氢睾酮的培养基中培养）的增殖不受培养基中睾酮浓度在10^(-6)-10^(-9) M范围内变化的影响。相比之下，睾酮改变了T家族细胞（在含有10^(-7) M睾酮的培养基中培养）的增殖，且这些作用可被抗雄激素RU 23908拮抗。初步研究表明，AXC/SSh大鼠前列腺癌细胞能分泌刺激体外细胞增殖的多肽成分。分泌这些成分的能力及其对体外细胞增殖的影响似乎都具有细胞系特异性。
