Yao Guo-Dong, Zhang Ya-Feng, Chen Peng, Ren Xiu-Bao
Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
Department of Biotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
Oncol Lett. 2018 Jan;15(1):991-997. doi: 10.3892/ol.2017.7381. Epub 2017 Nov 9.
Dysregulation of microRNAs has been confirmed to serve an important role in cancer development and progression. However, the role of microRNA (miR)-544 in colorectal cancer progression remains unknown. In the present study, it was observed that the expression level of miR-544 was increased in breast cancer cell lines and tissues using the quantitative polymerase chain reaction. Overexpression of miR-544 promoted cell proliferation and invasion in colorectal cancer, whereas inhibition of miR-544 suppressed colorectal cancer progression as determined using MTT, colony formation and Transwell assays. Furthermore, forkhead box O1 (FOXO1) was a direct target of miR-544. FOXO1 mediated miR-544-regulated colorectal cancer progression and cell cycle distribution. In conclusion, the results of the present study revealed that miR-544 serves an important role in promoting human colorectal cancer cell progression.
微小RNA的失调已被证实对癌症的发生和发展起着重要作用。然而，微小RNA（miR）-544在结直肠癌进展中的作用尚不清楚。在本研究中，通过定量聚合酶链反应观察到，miR-544在乳腺癌细胞系和组织中的表达水平升高。miR-544的过表达促进了结直肠癌的细胞增殖和侵袭，而使用MTT、集落形成和Transwell实验确定，抑制miR-544可抑制结直肠癌进展。此外，叉头框O1（FOXO1）是miR-544的直接靶点。FOXO1介导miR-544调控的结直肠癌进展和细胞周期分布。总之，本研究结果表明，miR-544在促进人类结直肠癌细胞进展中起重要作用。
