Lu Pengwei, Gu Yuanting, Li Lin, Wang Fang, Qiu Xinguang
Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Oncol Res. 2016;23(4):165-70. doi: 10.3727/096504016X14519157902726.
Accumulating evidence has reported the significant role of miRNAs in the underlying biology of tumors, including breast cancer. The purpose for this study was to investigate the potential effects of miR-544a in breast cancer migration and invasion. The human normal breast Hs578Bst cells and the human breast cancer MCF-7 and MDA-MB-231 cells were used to analyze the expression of miR-544a by RT-PCR. The effects of miR-544a on the two kinds of breast cancer cell migration and invasion were analyzed using the Matrigel and Transwell assay, respectively. miR-544a expression on the cell metastasis-related protein expression was also analyzed using Western blotting. Compared to the normal Hs578Bst cells, miR-544a was significantly downregulated in MCF-7 cells but was upregulated in MDA-MB-231 cells (p < 0.01). The overexpressed miR-544a significantly promotes the migrated and invaded MCF-7 cells (p < 0.05), which was opposite to that in MCA-MB-231 cells (p < 0.05). Moreover, the cadherin 1 (CDH1) expression was negatively correlated to miR-544a expression in the two kinds of cells. Our study suggested that the overexpressed miR-544a may be a promoter for breast cancer migration and invasion by targeting CDH1.
越来越多的证据表明，微小RNA（miRNA）在包括乳腺癌在内的肿瘤基础生物学中发挥着重要作用。本研究的目的是探讨miR-544a在乳腺癌迁移和侵袭中的潜在作用。使用人正常乳腺Hs578Bst细胞以及人乳腺癌MCF-7和MDA-MB-231细胞，通过逆转录聚合酶链反应（RT-PCR）分析miR-544a的表达。分别使用基质胶和Transwell实验分析miR-544a对两种乳腺癌细胞迁移和侵袭的影响。还使用蛋白质免疫印迹法分析miR-544a对细胞转移相关蛋白表达的影响。与正常Hs578Bst细胞相比，miR-544a在MCF-7细胞中显著下调，但在MDA-MB-231细胞中上调（p < 0.01）。过表达的miR-544a显著促进MCF-7细胞的迁移和侵袭（p < 0.05），这与MDA-MB-231细胞中的情况相反（p < 0.05）。此外，在这两种细胞中，钙黏蛋白1（CDH1）的表达与miR-544a的表达呈负相关。我们的研究表明，过表达的miR-544a可能通过靶向CDH1促进乳腺癌的迁移和侵袭。
