Fan Shengnuo, Habib Ahsan, Liu Jun, Tan Jun
Rashid Laboratory for Developmental Neurobiology, Silver Child Development Center, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South FloridaTampa, FL33613, USA.
Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen UniversityGuangzhou 510120, PR China.
Am J Transl Res. 2018 Jan 15;10(1):283-291. eCollection 2018.
Neuroinflammation is a complex pathological process usually results from abnormal microglial activation, thus, intervention in a microglial stimulation pathway could be a promising approach for the treatment of neurodegenerative diseases. Luteolin is an important bioflavonoid possesses anti-inflammatory properties, which is widely studied over these years. Light emitting diode (LED) therapy is reported to be a potential therapeutic strategy for many diseases including neurodegenerative diseases. However, little is known about the anti-inflammatory effect of LED therapy on activated microglial cells, even less is known whether there is a synergistic anti-inflammatory effect exist in LED and luteolin therapy. In this study, we aimed to confirm the anti-inflammatory effect of luteolin and LED combination therapy in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. We showed that luteolin inhibited LPS-induced cytotoxicity, tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) production through modulation of p38 and extracellular signal-regulated kinase (ERK) signaling in BV2 cells. In addition, LED therapy enhanced the anti-inflammatory effect of luteolin. These results suggest that a synergistic effect between luteolin and LED could be a new effective therapy in relieving neuroinflammation.
神经炎症是一种复杂的病理过程，通常由小胶质细胞异常激活引起，因此，干预小胶质细胞刺激途径可能是治疗神经退行性疾病的一种有前景的方法。木犀草素是一种具有抗炎特性的重要生物类黄酮，这些年来得到了广泛研究。据报道，发光二极管（LED）疗法是包括神经退行性疾病在内的许多疾病的一种潜在治疗策略。然而，关于LED疗法对活化小胶质细胞的抗炎作用知之甚少，对于LED和木犀草素联合疗法是否存在协同抗炎作用更是知之甚少。在本研究中，我们旨在证实木犀草素与LED联合疗法对脂多糖（LPS）刺激的BV2小胶质细胞的抗炎作用。我们发现木犀草素通过调节BV2细胞中的p38和细胞外信号调节激酶（ERK）信号传导，抑制LPS诱导的细胞毒性、肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）的产生。此外，LED疗法增强了木犀草素的抗炎作用。这些结果表明，木犀草素与LED之间的协同作用可能是缓解神经炎症的一种新的有效疗法。
