长链非编码RNA ZFAS1的沉默通过阻断胃癌细胞中的Wnt/β-连环蛋白信号传导来抑制恶性肿瘤。
Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells.
作者信息
Xu Weiran, He Liang, Li Ying, Tan Yan, Zhang Fan, Xu Hong
机构信息
a Department of Gastroenterology , The First Hospital of Jilin University , Changchun , People's Republic of China.
b Department of Gastrointestinal Surgery , The First Hospital of Jilin University , Changchun , People's Republic of China.
出版信息
Biosci Biotechnol Biochem. 2018 Mar;82(3):456-465. doi: 10.1080/09168451.2018.1431518. Epub 2018 Feb 9.
Gastric cancer is a common malignancy with high mortality. Long noncoding RNA (lncRNA) zinc finger antisense (ZFAS)1 is upregulated in gastric cancer specimens compared with the para-carcinoma tissues. The silencing of ZFAS1 inhibited the growth, proliferation, cell cycle progress, migration, invasion and epithelial-mesenchymal transition (EMT), and enhanced the sensitivity to cis-platinum or paclitaxel in SGC7901 cells, as evidenced by the expression changes of proliferating cell nuclear antigen, Cyclin D1, Cyclin E, Cyclin B1, E-cadherin, N-cadherin, vimentin, matrix metalloproteinase (MMP)-2 and MMP-14. The ZFAS1 also activated the Wnt/β-catenin signaling. Subsequently, the ZFAS1 knockdown-induced the inhibition of migration, invasion, EMT and resistance to chemotherapeutic reagens was reversed by the overexpression of β-catenin. In summary, the silencing of ZFAS1 inhibited the growth, proliferation, cell cycle progress, migration, invasion, EMT and chemotherapeutic tolerance by blocking the Wnt/β-catenin signaling in gastric cancer cells.
胃癌是一种常见的恶性肿瘤，死亡率很高。与癌旁组织相比，长链非编码RNA（lncRNA）锌指反义（ZFAS）1在胃癌标本中上调。ZFAS1的沉默抑制了SGC7901细胞的生长、增殖、细胞周期进程、迁移、侵袭和上皮-间质转化（EMT），并增强了对顺铂或紫杉醇的敏感性，增殖细胞核抗原、细胞周期蛋白D1、细胞周期蛋白E、细胞周期蛋白B1、E-钙黏蛋白、N-钙黏蛋白、波形蛋白、基质金属蛋白酶（MMP）-2和MMP-14的表达变化证明了这一点。ZFAS1还激活了Wnt/β-连环蛋白信号通路。随后，β-连环蛋白的过表达逆转了ZFAS1敲低诱导的迁移、侵袭、EMT抑制和对化疗药物的耐药性。总之，ZFAS1的沉默通过阻断胃癌细胞中的Wnt/β-连环蛋白信号通路，抑制了细胞的生长、增殖、细胞周期进程、迁移、侵袭、EMT和化疗耐受性。
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