Miranda Rosario, Farina Eleonora, Farina Michele A
Medical Angiologist, Specialista Ambulatoriale Branca di Angiologia - Azienda Sanitaria Locale NA3-Sud Distretto di Nola (NA), Italy.
Vascular Surgeon, Clinica Minerva - Santa Maria Capua Vetere (CE), Italy.
J Wound Care. 2018 Feb 2;27(2):60-65. doi: 10.12968/jowc.2018.27.2.60.
The Rigenera system is a new standardised micrograft preparation system. It works by means of automated mechanical disaggregation of small tissue samples, extracting only the smallest cells (<50µm). The aim of this study was to retrospectively evaluate patients affected by chronic ulcers and who were treated with the micrograft preparation method.
Chronic ulcers have been included regardless of the cause. The specimen was collected with a 3mm diameter biopsy punch and immediately dissociated by means of the Rigenera System. The obtained suspension was placed on a scaffold of equine collagen.
We included 15 patients (four males, 11 females) with a mean age of 72.2±8.41 (mean±standard deviation) years. In seven patients the ulcers were related to the complications of diabetes, post-traumatic in a further three diabetic patients, vasculitis in one patient, and four patients had venous leg ulcers (VLUs). The median main diameter was 5.0cm and the median estimated area was 43.96cm. The ulcers were present from a mean of 4.50±2.30 months before inclusion in this study. At the second week the wounds were reduced by 37.33%±19.35%, at the week eight, nine patients (60.0%) were healed, and at week 16, 13 (86.7%) were healed. The quality of scars was good and did not deteriorate at the six month follow-up.
The simplicity of the approach, the minimal invasiveness of the specimen collection, and the good quality of scarring of healed wounds, confirmed in the follow-up, makes this micrograft preparation method a useful tool to use on large or complex wounds.
Rigenera系统是一种新型标准化微移植制备系统。它通过对小组织样本进行自动机械解离来工作，仅提取最小的细胞（<50µm）。本研究的目的是回顾性评估受慢性溃疡影响并接受微移植制备方法治疗的患者。
纳入病因不限的慢性溃疡患者。用直径3mm的活检打孔器采集标本，并立即通过Rigenera系统进行解离。将获得的悬液置于马胶原蛋白支架上。
我们纳入了15例患者（4例男性，11例女性），平均年龄为72.2±8.41（平均值±标准差）岁。7例患者的溃疡与糖尿病并发症有关，另外3例糖尿病患者为创伤后溃疡，1例患者为血管炎，4例患者为下肢静脉溃疡（VLU）。溃疡的中位最大直径为5.0cm，中位估计面积为43.96cm²。在纳入本研究之前，溃疡平均已存在4.50±2.30个月。在第二周时，伤口缩小了37.33%±19.35%，在第八周时，9例患者（60.0%）愈合，在第16周时，13例（86.7%）愈合。瘢痕质量良好，在六个月的随访中没有恶化。
该方法操作简单，标本采集的侵入性极小，且愈合伤口的瘢痕质量良好，随访结果证实，这使得这种微移植制备方法成为处理大面积或复杂伤口的有用工具。
