di Summa Pietro G, Di Marzio Nicola, Jafari Paris, Jaconi Marisa E, Nesic Dobrila
Department of Plastic and Hand Surgery, University Hospital of Lausanne (CHUV), University of Lausanne (UNIL), 1015 Lausanne, Switzerland.
AO Research Institute Davos, 7270 Davos, Switzerland.
J Clin Med. 2023 Oct 16;12(20):6564. doi: 10.3390/jcm12206564.
Successful treatments for acute and chronic skin wounds remain challenging. The goal of this proof-of-concept study was to assess the technical feasibility and safety of a novel wound treatment solution, FastSkin, in a pig model. FastSkin was prepared from skin micrografts patterned in blood using acoustic waves. Upon coagulation, the graft was transferred on a silicone sheet and placed on wounds. Six full-thickness wounds were created at the back of two pigs and treated with either FastSkin, split-thickness skin graft (positive control), a gauze coverage (negative control, NC1), or blood patterned without micrografts (negative control, NC2). Silicone sheets were removed after 7, 14, and 21 days. Wound healing was monitored for six weeks and evaluated macroscopically for re-epithelialization and morphometrically for residual wound area and wound contraction. Tissue regeneration was assessed with histology after six weeks. Re-epithelialization was faster in wounds covered with FastSkin treatments compared to NC2 and in NC2 compared to NC1. Importantly, an enhanced collagen organization was observed in FastSkin in contrast to NC treatments. In summary, two clinically approved skin wound treatments, namely micrografting and blood clot graft, were successfully merged with sound-induced patterning of micrografts to produce an autologous, simple, and biologically active wound treatment concept.
急性和慢性皮肤伤口的成功治疗仍然具有挑战性。本概念验证研究的目的是在猪模型中评估一种新型伤口治疗解决方案FastSkin的技术可行性和安全性。FastSkin是由利用声波在血液中形成图案的皮肤微移植体制备而成。凝血后，将移植体转移到硅胶片上并放置在伤口上。在两只猪的背部制造六个全层伤口，分别用FastSkin、断层皮片移植（阳性对照）、纱布覆盖（阴性对照，NC1）或无微移植体的血液图案（阴性对照，NC2）进行治疗。7天、14天和21天后移除硅胶片。监测伤口愈合六周，并进行宏观评估以观察再上皮化情况，进行形态测量以评估残余伤口面积和伤口收缩情况。六周后通过组织学评估组织再生情况。与NC2相比，FastSkin治疗覆盖的伤口再上皮化更快，与NC1相比，NC2的再上皮化更快。重要的是，与NC治疗相比，FastSkin中观察到胶原组织增强。总之，两种临床批准的皮肤伤口治疗方法，即微移植和血凝块移植，成功地与声音诱导的微移植体图案化相结合，产生了一种自体、简单且具有生物活性的伤口治疗概念。
