Department of General Ward, Community Health Service Center of Laoshan District, Qingdao, Shandong, China; Medical College of Qingdao University, Qingdao, Shandong, China.
Eur Rev Med Pharmacol Sci. 2018 Jan;22(2):540-546. doi: 10.26355/eurrev_201801_14207.
Previous studies have demonstrated that microRNA-379 (miR-379) was involved in regulating cell proliferation. The present study aimed to investigate its potential role in the diagnosis of cute myocardium infarction (AMI).
Plasma samples from 30 patients with AMI and 30 healthy adults (non-AMI controls) were collected. The abundance of circulating miR-379 was determined using quantitative Real-time PCR (qRT-PCR). Receiver-operator characteristic (ROC) analyses were performed by GraphPad Prism 5.0 and the areas under the curve (AUC) were calculated. The proliferative ability and cell cycle progression of vascular smooth muscle cell (VSMC) were measured by CCK-8 and flow cytometry, respectively.
We found that the plasma miR-379 level was significantly decreased in patients with AMI compared with healthy people. Further studies demonstrated the miR-379 was negatively correlated with creatine kinase-MB (CK-MB) and cTns in study subjects. Finally, ROC analysis revealed an AUC value of 0.751 in discriminating AMI patients from healthy controls. Function assay in vitro further indicated miR-379 inhibited cell proliferation and induced cell cycle G0/G1 arrest in VSMCs.
Our results suggest that miR-379 may be a novel biomarker for the diagnosis of AMI by affecting VSMC cell function, which could be used in the early diagnosis of AMI.
先前的研究表明 microRNA-379（miR-379）参与调节细胞增殖。本研究旨在探讨其在急性心肌梗死（AMI）诊断中的潜在作用。
收集 30 例 AMI 患者和 30 例健康成年人（非 AMI 对照组）的血浆样本。采用实时定量 PCR（qRT-PCR）测定循环 miR-379 的丰度。通过 GraphPad Prism 5.0 进行接收者操作特征（ROC）分析，并计算曲线下面积（AUC）。通过 CCK-8 和流式细胞术分别测量血管平滑肌细胞（VSMC）的增殖能力和细胞周期进程。
我们发现 AMI 患者的血浆 miR-379 水平明显低于健康人。进一步的研究表明，miR-379 与研究对象中的肌酸激酶同工酶-MB（CK-MB）和 cTns 呈负相关。最后，ROC 分析显示区分 AMI 患者和健康对照组的 AUC 值为 0.751。体外功能测定进一步表明，miR-379 抑制 VSMC 细胞增殖并诱导细胞周期 G0/G1 期阻滞。
我们的结果表明，miR-379 可能通过影响 VSMC 细胞功能成为 AMI 诊断的新型生物标志物，可用于 AMI 的早期诊断。
